Unchanged total number of neurons in motor cortex and neocortex in amyotrophic lateral sclerosis: a stereological study

• Published in: Journal of neuroscience methods Vol. 95; no. 2; pp. 171 - 176
• Main Authors: Gredal, Ole, Pakkenberg, Henning, Karlsborg, Merete, Pakkenberg, Bente
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.02.2000; Elsevier Science
• Subjects: Aged; Amyotrophic lateral sclerosis; Amyotrophic Lateral Sclerosis - pathology; Biological and medical sciences; Brain volumes; Cell Count; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Female; Humans; Male; Medical sciences; Middle Aged; Motor cortex; Motor Cortex - pathology; Neocortex; Neocortex - pathology; Neurology; Neurons - pathology; Quantification; Stereology; Amyotrophic lateral sclerosis; Stereology; Quantification; Neocortex; Brain volumes; Motor cortex; Human; Nervous system diseases; Cerebral cortex; Methodology; Motor pathway; Central nervous system; Quantization; Genetic disease; Neuron; Investigation method; Central nervous system disease; Degenerative disease; Measurement method; Spinal cord disease; Comparative study; Brain (vertebrata)
• ISSN: 0165-0270; 1872-678X
• DOI: 10.1016/S0165-0270(99)00175-2

Modern stereological methods provide precise and reliable estimates of the number of neurons in specific regions of the brain. The total number of neurons in the neocortex and motor cortex from eight patients suffering from amyotrophic lateral sclerosis (ALS) and nine controls was estimated. No attempt was made to estimate subpopulations of neurons such as the number of giant pyramidal cells of Betz. No difference was found in the average number of neurons in neocortex in ALS and controls, 21.7 and 22.3×10 9, respectively, and 1.33 and 1.29×10 9 in motor cortex, respectively. In the light of our stereological measurements, results obtained from in-vivo proton magnetic resonance spectroscopy ( 1H-MRS), suggesting neuronal loss in ALS, may instead be due to neuronal metabolic dysfunction and/or alteration in the size or the volume fraction of the neurons.