Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus

• Published in: Bioprocess and biosystems engineering Vol. 42; no. 6; pp. 953 - 961
• Main Authors: Maldonado, S. Lladó, Krull, J., Rasch, D., Panjan, P., Sesay, A. M., Marques, M. P. C., Szita, N., Krull, R.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2019; Springer Nature B.V
• Subjects: Aeration; Biomass; Bioreactors; Bioreactors - microbiology; Biotechnology; Chemistry; Chemistry and Materials Science; Cultivation; Data processing; Dilution; Environmental Engineering/Biotechnology; Food Science; Glucose; Growth rate; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Kinetics; Nozzles; Polystyrene; Polystyrene resins; Reaction kinetics; Reagents; Research Paper; Staphylococcus - growth & development; Staphylococcus carnosus; Substrates; Microbioreactor; Microbubble column bioreactor; Chemostat; Reaction kinetics; Staphylococcus carnosus
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-019-02095-9

Bioreactors at the microliter scale offer a promising approach to accelerate bioprocess development. Advantages of such microbioreactors include a reduction in the use of expensive reagents. In this study, a chemostat operation mode of a cuvette-based microbubble column bioreactor made of polystyrene (working volume of 550 µL) was demonstrated. Aeration occurs through a nozzle ( Ø  ≤ 100 µm) and supports submerged whole-cell cultivation of Staphylococcus carnosus . Stationary concentrations of biomass and glucose were determined in the dilution rate regime ranging from 0.12 to 0.80 1/h with a glucose feed concentration of 1 g/L. For the first time, reaction kinetics of S. carnosus were estimated from data obtained from continuous cultivation. The maximal specific growth rate ( µ max  = 0.824 1/h), Monod constant ( K S  = 34 × 10 − 3 g S /L), substrate-related biomass yield coefficient ( Y X/S  = 0.315 g CDW /g S ), and maintenance coefficient ( m S  = 0.0035 g S /(g CDW ·h)) were determined. These parameters are now available for further studies in the field of synthetic biology.