Maternal KIR repertoire is not associated with recurrent spontaneous abortion

• Published in: Human reproduction (Oxford) Vol. 19; no. 11; pp. 2653 - 2657
• Main Authors: Witt, C.S., Goodridge, J., Gerbase-DeLima, M.G., Daher, S., Christiansen, F.T.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.11.2004; Oxford Publishing Limited (England)
• Subjects: Abortion, Habitual - genetics; Adolescent; Adult; Biological and medical sciences; Case-Control Studies; Embryology: invertebrates and vertebrates. Teratology; Female; Fundamental and applied biological sciences. Psychology; Gene Frequency; Genetic Predisposition to Disease; Humans; killer cell immunoglobulin-like receptors; Middle Aged; NK cell; Pregnancy; Receptors, Immunologic - blood; Receptors, Immunologic - genetics; Receptors, KIR; Receptors, KIR2DL1; Receptors, KIR2DL4; recurrent spontaneous abortion; killer cell immunoglobulin-like receptors; recurrent spontaneous abortion; NK cell; Natural killer cell; Vertebrata; Immunoglobulins; Relapse; Mammalia; Pregnancy disorders; Mother; Killer cell; Abortion; Biological receptor; killer cell immunoglobulin-like receptors/NK cell/recurrent spontaneous abortion
• ISSN: 0268-1161; 1460-2350
• DOI: 10.1093/humrep/deh483

BACKGROUND: In view of evidence suggesting an immunological cause of recurrent spontaneous abortions (RSA) and the large number of maternal natural killer (NK) cells present in the pregnant uterus, we investigated the genetic polymorphism of the killer cell immunoglobulin-like receptors (KIR) in women with RSA. METHODS: KIR gene repertoire and KIR2DL4 (a receptor for HLA-G) genotyping were determined by SSP and SSCP respectively, in women experiencing RSA and controls. RESULTS: The KIR repertoire did not differ between RSA patients and controls in terms of: (i) the number of inhibitory receptors; (ii) the number of activating receptors; (iii) the ratio of inhibitory to activating receptors. KIR2DL4, a receptor for HLA-G, has different transmembrane alleles, which produce functionally different phenotypes. The frequency of KIR2DL4 transmembrane genotypes differed significantly between RSA patients and controls (P=0.03). However, although homozygosity for a membrane-bound receptor was more frequent in patients (25%) than controls (10%), other genotypes that would produce the same phenotype were not more frequent in patients than controls. CONCLUSIONS: The data provide little evidence that KIR polymorphism plays a role in predisposition to RSA.