Transmission of antimicrobial resistant non-O157 Escherichia coli at the interface of animal-fresh produce in sustainable farming environments

• Published in: International journal of food microbiology Vol. 319; p. 108472
• Main Authors: Glaize, Ayanna, Gutierrez-Rodriguez, Eduardo, Hanning, Irene, Díaz-Sánchez, Sandra, Gunter, Chris, van Vliet, Arnoud H.M., Watson, Wes, Thakur, Siddhartha
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16.04.2020; Elsevier BV
• Subjects: Ampicillin; Antiinfectives and antibacterials; Antimicrobial agents; Antimicrobial resistance; E coli; Farm management; Farming; Farms; Foodborne diseases; Gene sequencing; Genomes; Non-O157 E. coli; Pathogen transmission; Pathogens; Phylotyping; Streptomycin; Stx gene; Sulfisoxazole; Sustainable agriculture; Sustainable production systems; Virulence; Pathogen transmission; Non-O157 E. coli; Phylotyping; Antimicrobial resistance; Sustainable production systems
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2019.108472

The interaction of typical host adapted enteric bacterial pathogens with fresh produce grown in fields is complex. These interactions can be more pronounced in co-managed or sustainable farms where animal operations are, by design, close to fresh produce, and growers frequently move between the two production environments. The primary objectives of this study were to 1) determine the transmission of STEC or enteric pathogens from small and large animal herds or operations to fresh produce on sustainable farms in TN and NC, 2) identify the possible sources that impact transmission of AMR E. coli, specifically STEC on these systems, and 3) WGS to characterize recovered E. coli from these sources. Samples were collected from raw and composted manure, environment, and produce sources. The serotype, virulence, and genotypic resistance profile were determined using the assembled genome sequences sequenced by Illumina technology. Broth microdilution was used to determine the antimicrobial susceptibility of each isolate against a panel of fourteen antimicrobials. The prevalence of E. coli increased during the summer season for all sources tested. ParSNP trees generated demonstrated that the transmission of AMR E. coli is occurring between animal feeding operations and fresh produce. Ten isolates were identified as serotype O45, a serotype that is associated with the “Big Six” group that is frequently linked with foodborne outbreaks caused by non-O157 E. coli. However, these isolates did not possess the stx gene. The highest frequency of resistance was detected against streptomycin (n = 225), ampicillin (n = 190) and sulfisoxazole FIS (n = 140). A total of 35 (13.7%) isolates from two TN farms were positive for the blaCMY (n = 5) and blaTEM (n = 32) genes. The results of this study show the potential of AMR E. coli transmission between animal feeding operations and fresh produce, and more studies are recommended to study this interaction and prevent dissemination in sustainable farming systems. •First to study E. coli transmission on commercial sustainable farms in two states•Transmission of AMR E. coli detected on sustainable farms.•Transmission was observed mainly during the summer months.•The majority (55%) of the E. coli were multidrug resistant.•WGS is a tool used to elucidate connections between isolates from multiple sources.