Evaluation of column bleed by using an ultraviolet and a charged aerosol detector coupled to a high-temperature liquid chromatographic system

• Published in: Journal of Chromatography A Vol. 1119; no. 1; pp. 197 - 201
• Main Authors: Teutenberg, T., Tuerk, J., Holzhauser, M., Kiffmeyer, T.K.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 30.06.2006; Elsevier
• Subjects: Aerosols; Analytical chemistry; Charged aerosol detection; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - standards; Exact sciences and technology; High-temperature HPLC; Hot Temperature; HPLC; Hydrogen-Ion Concentration; Online Systems; Other chromatographic methods; Stationary phase stability; Temperature programming; Ultraviolet detection; Ultraviolet Rays; Temperature programming; Ultraviolet detection; High-temperature HPLC; Charged aerosol detection; Stationary phase stability; HPLC; Evaluation; Coupled method; HPLC chromatography; Thermal stability; Operating conditions; Programmed temperature; Particle detection; Ultraviolet detector; Charged particle; Aerosols; Stationary phase
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2005.12.011

In this study, five different HPLC columns were heated to 200 °C using a homemade heating system which can be operated in temperature programmed mode. The column bleed as an indicator of induced degradation of the stationary phase material was evaluated using a charged aerosol detector (CAD) and an ultraviolet diode array detector (UV-DAD) at different wavelengths. The silica based C-18 stationary phase gave the highest bleed, and the carbon clad titanium dioxide column the lowest bleed. This was independent of both the detection technique and the wavelength.