Amounts and proportion of administered pyrene dose excreted as urinary 1-hydroxypyrene after dietary exposure to polycyclic aromatic hydrocarbons

• Published in: Archives of toxicology Vol. 84; no. 10; pp. 767 - 776
• Main Authors: Chien, Yeh-Chung, Yeh, Chun-Ting
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.10.2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Adult; Atmosphere - chemistry; Biological and medical sciences; Biomarkers - urine; Biomedical and Life Sciences; Biomedicine; Carcinogenesis, carcinogens and anticarcinogens; Chemical agents; Environmental Exposure - analysis; Environmental Health; Environmental Pollutants - urine; Female; Fluorenes - analysis; Grills; Humans; Male; Meat; Meat - analysis; Medical sciences; Metabolism; Occupational Medicine/Industrial Medicine; Pharmacology/Toxicology; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - analysis; Polycyclic Aromatic Hydrocarbons - urine; Pyrenes - analysis; Pyrenes - metabolism; Toxicokinetics and Metabolism; Toxicology; Tumors; Urinalysis; Young Adult; Barbecued meats; Excretion kinetics; PAH; 1-Hydroxypyrene; Urine; Excretion; Hydrocarbon; Nutrition; Exposure; Polycyclic aromatic compound; Feeding; Meat; Carcinogen; Pyrene; Diet; Proportion; Kinetics; Dose; Food
• ISSN: 0340-5761; 1432-0738; 1432-0738
• DOI: 10.1007/s00204-010-0570-4

Although urinary 1-hydroxypyrene (1-OHP) is the most relevant parameter for assessing exposure to polycyclic aromatic hydrocarbons, the inability to further elucidate the intra- and inter-individual variability, specificity and kinetics makes it difficult to enhance its value as an exposure predictor. Therefore, this human control study examined the excretion kinetics of urinary 1-OHP after consuming barbecued meat. Two feeding experiments were conducted, with doses of 15 and 30 g of barbecued meat per kg of body weight for experiments 1 and 2, respectively. All voided urine was collected for 7 days and analyzed for 1-OHP. In both experiments, the amounts of urinary 1-OHP excreted was significantly increased (P < 0.05) at 12 h post exposure but not at 12-24 h post exposure. Mean percentages of administered pyrene doses excreted as urinary 1-OHP at 0-12 h and 12-24 h post exposure were 3.80 and 0.61% in experiment 1 and 1.66 and 0.38% in experiment 2. Excretion ratio was inversely related to dose. A pattern of diurnal fluctuation (P < 0.05) in 1-OHP excretions was also identified. That is, 1-OHP excretions were smaller in the first half of the day (~0:00-12:00) than in the last half of the day (~12:00-24:00). This study demonstrated that, even at large dietary doses, most of the total urinary excretion of 1-OHP occurs within 12 h. Thus, subjects of occupational or environmental studies need only recall their diets for the current or previous day to diminish the influence from dietary pyrene.