Enhancement of serotonin- and bradykinin-evoked calcium ion dynamics in differentiated NG108-15 cells

• Published in: Neuroscience letters Vol. 405; no. 1; pp. 1 - 4
• Main Authors: Imanishi, Takashi, Matsushima, Kayoko, Kawaguchi, Akinori, Wada, Tetsuyuki, Yoshida, Shigeru, Ichida, Seiji
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 11.09.2006; Elsevier
• Subjects: Acetylcholine - pharmacology; Acetylcholine - physiology; Animals; Biological and medical sciences; Bradykinin; Bradykinin - pharmacology; Bradykinin - physiology; Calcium - metabolism; Cations, Divalent; Cell Differentiation; Cell Line, Tumor; Differentiation; Fundamental and applied biological sciences. Psychology; Glioma; Glutamic Acid - pharmacology; Glutamic Acid - physiology; Hybrid Cells; Intracellular Space - metabolism; Intracellular-calcium ion concentration; Mice; Neuroblastoma; Neurotransmitter Agents - pharmacology; Neurotransmitter Agents - physiology; Neurotransmitter candidates; NG108-15 cells; Norepinephrine - pharmacology; Norepinephrine - physiology; Rats; Serotonin; Serotonin - pharmacology; Serotonin - physiology; Vertebrates: nervous system and sense organs; Intracellular-calcium ion concentration; NG108-15 cells; Serotonin; Differentiation; Bradykinin; Neurotransmitter candidates; Hybrid cell; Rat; Calcium; Rodentia; Neuropeptide; Vertebrata; Mammalia; Mouse; Animal; Neurotransmitter; Intracellular
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2005.12.035

Dynamic changes in the concentration of intracellular free-calcium ion ([Ca 2+] i) by carbachol (CCh) and neurotransmitter candidates was investigated in undifferentiated and differentiated neuroblastoma × glioma hybrid NG108-15 (NG) cells. [Ca 2+] i was increased in a dose-dependent manner by bradykinin (BK) and serotonin (5-HT) in differentiated NG cells, and the response to BK and 5-HT was significantly greater than that in undifferentiated NG cells. The EC 50 value of BK was approximately 1.5 × 10 −8 M in both undifferentiated and differentiated NG cells. The EC 50 value of 5-HT in differentiated NG cells was about 5 × 10 −6 M. The response to BK and 5-HT was almost completely inhibited by 10 nM Hoe140 (a BK B2 receptor antagonist) and 3 nM tropisetron (a 5-HT 3 receptor antagonist), respectively. These results suggest that there are some mechanisms by which the response evoked by BK and 5-HT is up-regulated in differentiated NG cells.