Preparation, characterization and in vitro release study of carvacrol-loaded chitosan nanoparticles
[Display omitted] ▶ Carvacrol-loaded chitosan particles were prepared through emulsion formation and ionic gelation. ▶ Particles exhibited spherical shape, 40-80nm-size and positively charged surfaces. ▶ Particles showed antimicrobial activity against S. aureus, B. cereus and E. coli. ▶ Release of c...
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Published in | Colloids and surfaces, B, Biointerfaces Vol. 84; no. 1; pp. 163 - 171 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.05.2011
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Subjects | |
Online Access | Get full text |
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Summary: | [Display omitted]
▶ Carvacrol-loaded chitosan particles were prepared through emulsion formation and ionic gelation. ▶ Particles exhibited spherical shape, 40-80nm-size and positively charged surfaces. ▶ Particles showed antimicrobial activity against S. aureus, B. cereus and E. coli. ▶ Release of carvacrol in an acidic solution was faster than alkaline and neutral media.
The fabrication of carvacrol-loaded chitosan nanoparticles was achieved by a two-step method, i.e., oil-in-water emulsion and ionic gelation of chitosan with pentasodium tripolyphosphate. The obtained particles possessed encapsulation efficiency (EE) and loading capacity (LC) in the ranges of 14–31% and 3–21%, respectively, when the initial carvacrol content was 0.25–1.25g/g of chitosan. The individual particles exhibited a spherical shape with an average diameter of 40–80nm, and a positively charged surface with a zeta potential value of 25–29mV. The increment of initial carvacrol content caused a reduction of surface charge. Carvacrol-loaded chitosan nanoparticles showed antimicrobial activity against Staphylococcus aureus, Bacillus cereus and Escherichia coli with an MIC of 0.257mg/mL. The release of carvacrol from chitosan nanoparticles reached plateau level on day 30, with release amounts of 53% in acetate buffer solution with pH of 3, and 23% and 33% in phosphate buffer solutions with pH of 7 and 11, respectively. The release mechanism followed a Fickian behavior. The release rate was superior in an acidic medium to either alkaline or neutral media, respectively. |
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Bibliography: | http://dx.doi.org/10.1016/j.colsurfb.2010.12.031 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0927-7765 1873-4367 |
DOI: | 10.1016/j.colsurfb.2010.12.031 |