Effects of Mycoplasma hyopneumoniae on porcine nasal cavity dendritic cells

• Published in: Veterinary microbiology Vol. 198; pp. 1 - 8
• Main Authors: Shen, Yumeng, Hu, Weiwei, Wei, Yanna, Feng, Zhixin, Yang, Qian
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2017; Elsevier BV
• Subjects: Animals; Antigen-presenting cells; Antigens; CD11b antigen; Cell Count; Cell Proliferation; Cells, Cultured; Cytokines - genetics; Dendritic cells; Dendritic Cells - immunology; Dendritic Cells - microbiology; Dendritic Cells - pathology; Enzootic pneumonia; Epithelium; Exposure; Gene Expression Regulation - immunology; Host-Pathogen Interactions - immunology; Immune response; Immunoglobulin A, Secretory - metabolism; Infections; Interferon; Interleukin 10; Interleukin 12; Livestock; Lymphocytes; Lymphocytes T; Macrophages - cytology; Mycoplasma; Mycoplasma hyopneumoniae; Mycoplasma hyopneumoniae - immunology; Nasal cavity; Nasal Cavity - immunology; Nasal Cavity - microbiology; Nasal Cavity - pathology; Nose; Pneumonia; Pneumonia of Swine, Mycoplasmal - immunology; Pneumonia of Swine, Mycoplasmal - pathology; Respiratory tract; Specific Pathogen-Free Organisms; Subpopulations; Swine; Swine enzootic pneumonia; T-Lymphocytes - cytology; T-Lymphocytes - immunology; γ-Interferon; Mycoplasma hyopneumoniae; Dendritic cells; Nasal cavity
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2016.11.018

•This study is the first description of the interaction between Mycoplasma hyopneumoniae (Mhp)and porcine DCs in vitro.•The number of DC and T cell populations decrease in nasal cavity after Mhp infection.•Phenotypic alteration of BMDCs stimulated by Mhp in vitro, Mhp 168 stimulate-BMDCs inhibite T cells proliferation. Mycoplasma hyopneumoniae (Mhp) is the primary etiological agent responsible for swine enzootic pneumonia (EP), a disease that cause tremendous economic losses all over the swine industry. Dendritic cells (DCs), the most effective antigen-presenting cells, are widely distributed beneath respiratory epithelium. DCs uptake and present antigens to T cells, to initiate protective immune responses or generate immune-mediated pathology in different infections. In this study, we investigated the changes in the different DCs subpopulations, T cells and SIgA positive cells counts in porcine nasal cavity after long time Mhp infection. We further evaluated the role of porcine DCs in Mhp exposure. Our results showed that the number of SLA-II-DR+SWC3a+DCs, SLA-II-DR+CD11b+ DCs, T cells, SIgA positive cells in nasal cavity were decreased after Mhp 28 days infection in vivo experiment. The antigen presenting ability of DCs were inhibited by Mhp exposure. DCs couldn’t activate T-cell proliferation by down-regulating the antigen presenting molecule CD1a expression and promoting high level of IL-10 production. Further more, the expression levels of IL-12 and IFN-γ in DCs were decreased, suggesting that DCs favour for Th2 immune response development after Mhp exposure in vitro. Taken together, Mhp infection impairs the immune function which allows the persistence of Mhp and cause predispose pigs to secondary infections. The decline of DCs presentation ability is the reason why dysfunction and persistence in Mhp infection. These findings are benefit for exploring the pathogenic mechanisms of Mhp in pigs.