Application of microfluidic chip with integrated optics for electrophoretic separations of proteins

• Published in: Journal of chromatography. B Vol. 845; no. 2; pp. 218 - 225
• Main Authors: Vieillard, Julien, Mazurczyk, Radoslaw, Morin, Christophe, Hannes, Benjamin, Chevolot, Yann, Desbène, Paul-Louis, Krawczyk, Stanislas
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 15.01.2007; Elsevier Science; Elsevier
• Subjects: Analysis; Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Capillary electrophoresis; Carbocyanines - isolation & purification; Catalysis; Chemical Sciences; Cheminformatics; Electroosmosis; Electrophoresis, Capillary - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; Integrated optics; Lab-on-a-chip; Medical sciences; Medicinal Chemistry; Microfluidic; Microfluidic Analytical Techniques - methods; Optics and Photonics - instrumentation; or physical chemistry; Organic chemistry; Pharmacology. Drug treatments; Proteins - isolation & purification; Reproducibility of Results; Streptavidin - isolation & purification; Theoretical and; Integrated optics; Lab-on-a-chip; Capillary electrophoresis; Microfluidic; Electrophoretic mobility; System on a chip; Protein
• ISSN: 1570-0232; 0378-4347; 1873-376X
• DOI: 10.1016/j.jchromb.2006.08.009

This paper describes the fabrication, the characterization and the applications of a capillary electrophoresis microchip. This hybrid device (glass/PDMS) features channels and optical waveguides integrated in one common substrate. It can be used for electrophoretic separation and fluorimetric detection of molecules. The microfluidic performance of the device is demonstrated by capillary zone and gel electrophoresis of proteins.