Water-soluble ZnCuInSe quantum dots for bacterial classification, detection, and imaging

• Published in: Analytical and bioanalytical chemistry Vol. 412; no. 30; pp. 8379 - 8389
• Main Authors: Geng, Hongchao, Qiao, Yan, Jiang, Ning, Li, Chenyi, Zhu, Xingqi, Li, Weili, Cai, Qingyun
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2020; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Bacteria; Biochemistry; Characterization and Evaluation of Materials; Chemical properties; Chemistry; Chemistry and Materials Science; Classification; Comparative analysis; Fluorescence; Fluorescent indicators; Food Science; Gram-negative bacteria; Gram-positive bacteria; Health risks; Heavy metals; Identification and classification; Imaging; Laboratory Medicine; Methods; Microscope and microscopy; Monitoring/Environmental Analysis; Optical properties; Photoluminescence; Photons; Quantum dots; Research Paper; Solution (Chemistry); Spectra; Structure; China; ZnCuInSe QDs; Bacterial classification; Detection; Imaging
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-020-02974-1

Bacteria are everywhere and pose severe threats to human health and safety. The rapid classification and sensitive detection of bacteria are vital steps of bacterial community research and the treatment of infection. Herein, we developed optical property–superior and heavy metal–free ZnCuInSe quantum dots (QDs) for achieving rapid discrimination of Gram-positive/Gram-negative bacteria by the naked eye; driven by the structural differences of bacteria, ZnCuInSe QDs are effective in binding to Gram-positive bacteria, especially Staphylococcus aureus ( S . aureus ), in comparison with Gram-negative bacteria and give discernable color viewed by the naked eye. Meanwhile, based on its distinctive fluorescence response, the accurate quantification of S . aureus was investigated with a photoluminescence system in the concentration ranges of 1 × 10 3 to 1 × 10 11  CFU/mL, with a limit of detection of 1 × 10 3  CFU/mL. Furthermore, we demonstrated the feasibility of ZnCuInSe QDs as a fluorescence probe for imaging S . aureus . This simple strategy based on ZnCuInSe QDs provides an unprecedented step for rapid and effective bacterial discrimination, detection, and imaging.