Human Papillomavirus Type 33 Polymorphisms and High-Grade Squamous Intraepithelial Lesions of the Uterine Cervix

• Published in: The Journal of infectious diseases Vol. 194; no. 7; pp. 886 - 894
• Main Authors: Khouadri, Soraya, Villa, Luisa L., Gagnon, Simon, Koushik, Anita, Richardson, Harriet, Ferreira, Silvaneide, Tellier, Pierre, Simao, João, Matlashewski, Greg, Roger, Michel, Franco, Eduardo L., Coutlée, Francois
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.10.2006; University of Chicago Press
• Subjects: Binding sites; Biological and medical sciences; Biological markers; Case-Control Studies; Cervical cancer; Cervical Intraepithelial Neoplasia - virology; Cervix uteri; Cervix Uteri - virology; Cohort Studies; Ethnicity; Female; Fundamental and applied biological sciences. Psychology; Genetic variation; Human papillomavirus; Human papillomavirus 16; Humans; Infections; Infectious diseases; Lesions; Medical sciences; Microbiology; Miscellaneous; Neoplasms, Squamous Cell - virology; Oncogene Proteins, Viral - genetics; Papillomaviridae - classification; Papillomaviridae - genetics; Papillomavirus Infections - virology; Polymerase Chain Reaction - methods; Polymorphism, Genetic; Sequence Analysis, DNA; Uterine Cervical Neoplasms - virology; Virology; Viruses; Papillomavirus; Virus; Infection; Human papillomavirus; Microbiology; Squamous intraepithelial lesion; Papovaviridae; Uterine cervix; Polymorphism
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/507431

BackgroundWe investigated the association between polymorphisms of human papillomavirus (HPV)–33 and squamous intraepithelial lesions (SILs) MethodsEndocervical specimens from 89 women infected with HPV-33, out of a total of 5347 recruited for 2 case-control and 2 cohort studies, were further analyzed by polymerase chain reaction sequencing of the long control region (LCR), E6, and E7 ResultsOf the 89 samples, 64 were normal, 7 had low-grade SILs (including 3 determined by histopathologic analysis), 15 had high-grade SILs (HSILs, including 14 determined by histopathologic analysis), and 3 had an unknown diagnosis. Non–prototype-like LCR variants were significantly associated with HSILs (age- and study site–adjusted odds ratio [OR], 9.2 [95% confidence interval {CI}, 1.8–45.9]). The C7732G variation, which results in the loss of a putative binding site for the cellular upstream stimulatory factor, was associated with HSILs (age- and site-adjusted OR, 8.0 [95% CI, 1.5–42.8]). E6 and E7 polymorphisms were not associated with HSILs. Samples collected at 6-month intervals from 14 participants contained the same variant. The HPV-33 MT 1-0-0 variant carrying the G7584A variation was detected more frequently in women from Brazil (7/20 [35%]) than in women from Canada (1/65 [1.5%]; P=.001) ConclusionIntratypic LCR variants of HPV-33 seem to vary geographically and to differ with respect to their oncogenic potential