Proteomics technologies for biomarker discovery in multiple sclerosis

• Published in: Journal of neuroimmunology Vol. 248; no. 1; pp. 40 - 47
• Main Authors: Singh, Vaibhav, Hintzen, Rogier Q, Luider, Theo M, Stoop, Marcel P
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.07.2012
• Subjects: Allergy and Immunology; Animals; Biomarker; biomarkers; Biomarkers - blood; Biomarkers - cerebrospinal fluid; Biomarkers - urine; Cerebrospinal fluid; Disease Models, Animal; Forecasting; Humans; Inflammatory diseases; Mass spectrometry; Mass Spectrometry - methods; Mass Spectrometry - trends; Mass spectroscopy; Molecular Diagnostic Techniques - methods; Molecular Diagnostic Techniques - trends; Multiple sclerosis; Multiple Sclerosis - diagnosis; Multiple Sclerosis - immunology; Multiple Sclerosis - metabolism; Neurodegenerative diseases; Neurology; Prognosis; Proteomics; Proteomics - methods; Proteomics - trends; Technologies; Multiple sclerosis; Biomarker; BBB; CNS; MS/MS; SELDI; SRM; isotope coded affinity tags; 2-DGE; experimental autoimmune encephalomyelitis; tandem mass spectrometry; CSF; mass spectrometry; ITRAQ; MS; LC–MS; EAE; surface enhanced laser desorption/ionization; liquid chromatography; isobaric tags for relative abundance and quantitation; selective reaction monitoring; central nervous system; ICAT; Technologies; blood brain barrier; cerebrospinal fluid; LC; Proteomics; liquid chromatography–mass spectrometry; 2-dimensional gel electrophoresis; Cerebrospinal fluid; Mass spectrometry
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/j.jneuroim.2011.11.004

Abstract Multiple sclerosis is a disabling inflammatory and neurodegenerative disorder that predominantly affects young adults. There is a great need for biomarkers, which could elucidate pathology as well as provide prognosis of disease progression and therapy response in multiple sclerosis. Rapidly evolving, technology driven applications such as mass spectrometry based proteomics are currently being developed for this purpose. In this review, we will outline the current status of the field and detail a number of the bottlenecks as well as future prospects of this type of biomarker research.