A Novel Strain of Bacillus cereus with a Strong Antagonistic Effect Specific to Sclerotinia and Its Genomic and Transcriptomic Analysis

• Published in: Microorganisms (Basel) Vol. 12; no. 3; p. 611
• Main Authors: Ma, Wanfu, Ding, Jinhao, Jia, Qingyun, Li, Qianru, Jiao, Shanhai, Guo, Xupeng, Fan, Chengming, Chen, Yuhong, Hu, Zanmin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19.03.2024; MDPI
• Subjects: antagonistic effect; Antifungal activity; Bacillus cereus; Bacteria; biocontrol; Biological control; Crop diseases; Crops; Disease control; Fermentation; Fungi; Fungicides; Genomics; Glucose; multiomics analysis; Oilseed crops; oilseed rape; Oilseeds; Pathogens; Physiology; Plant diseases; Rape plants; Rapeseed; Rhizosphere; Sclerotinia sclerotiorum; Transcriptomics; China; Sclerotinia sclerotiorum; Bacillus cereus; multiomics analysis; biocontrol; antagonistic effect; oilseed rape
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms12030611

Sclerotinia, which is caused by , is a severe disease of oilseed rape, which is an important oil crop worldwide. In this study, we isolated a novel strain of , named HF10, from the rhizosphere soil of the reed on the seaside of Yagzhou Bay, Sanya city, Hainan Province, China. HF10 exhibited a significant antagonistic effect on , with an inhibition rate of 79%, and to other species in , but no antagonistic effect was found on various other fungi or bacteria. HF10 had an 82.3% inhibitory effect on the infection of oilseed rape leaves and a 71.7% control effect on Sclerotinia infection in oilseed rape based on in vitro and in vivo experiments, respectively. The genomics and transcriptomics of HF10 and its loss of the antifungal function mutant Y11 were analyzed, and the results provided insight into potential antifungal substances. Our work provides a novel strain, HF10, for developing a promising biological control agent against , which infects oilseed rape and other plants.