Site-Specific Cleavage of Single-Stranded DNA by a Hemophilus Restriction Endonuclease

Single-stranded viral DNA of bacteriophage f1 is cleaved into specific fragments by endo R· HaeIII, a restriction endonuclease isolated from Hemophilus aegyptius. The sites of the single strand cleavage correspond to those of the double strand cleavage. A single-stranded DNA fragment containing only...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 72; no. 7; pp. 2555 - 2558
Main Authors Horiuchi, Kensuke, Zinder, Norton D.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.07.1975
National Acad Sciences
Subjects
Bacteriophages
Base Sequence
Biochemistry
Chromosome Mapping
Coliphages
DNA
DNA Restriction Enzymes - metabolism
DNA Viruses
DNA, Single-Stranded
DNA, Viral
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Endonucleases
Enzymes
Gels
Haemophilus
Haemophilus - enzymology
Nucleic Acid Denaturation
Nucleic Acid Renaturation
Nucleotide sequences
Single stranded DNA
Viral DNA
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Summary:Single-stranded viral DNA of bacteriophage f1 is cleaved into specific fragments by endo R· HaeIII, a restriction endonuclease isolated from Hemophilus aegyptius. The sites of the single strand cleavage correspond to those of the double strand cleavage. A single-stranded DNA fragment containing only one HaeIII site is also cleaved by this enzyme. This observation suggests that the reaction of single-stranded DNA cleavage does not require the formation of a symmetrical double-stranded structure that would result from the intramolecular base-pairing between two different HaeIII sites. Other restriction endonucleases may also cleave single-stranded DNA.
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.72.7.2555