Bioaugmented hydrogen production from carboxymethyl cellulose and partially delignified corn stalks using isolated cultures

Dark fermentation of carboxymethyl cellulose to produce biohydrogen using mono-culture or co-culture of isolated strains was studied. Three isolates were noted to effectively hydrolyze cellulosic substrates and degrade the metabolites to hydrogen and volatile fatty acids. The strain Clostridium acet...

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Published inInternational journal of hydrogen energy Vol. 33; no. 19; pp. 5250 - 5255
Main Authors Ren, Nanqi, Wang, Aijie, Gao, Lingfang, Xin, Liang, Lee, Duu-Jong, Su, Ay
Format Journal Article Conference Proceeding
LanguageEnglish
Published Kidlington Elsevier Ltd 01.10.2008
Elsevier
Subjects
Alternative fuels. Production and utilization
Applied sciences
Biohydrogen
Cellulose
Co-culture
Corn stalks
Delignification
Energy
Exact sciences and technology
Fuels
Hydrogen
Delignification
Biohydrogen
Co-culture
Corn stalks
Cellulose
Clostridium
Biotechnology
Clostridiales
Explosions
Fermentation
Hydrolysis
Clostridiaceae
Bacteria
Corn by product
Pretreatment
Hydrogen production
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Summary:Dark fermentation of carboxymethyl cellulose to produce biohydrogen using mono-culture or co-culture of isolated strains was studied. Three isolates were noted to effectively hydrolyze cellulosic substrates and degrade the metabolites to hydrogen and volatile fatty acids. The strain Clostridium acetobutylicum X 9 was noted to have the highest hydrogen yield amongst the three isolates in all tests. Co-cultures of any two of the three isolates and with another strain Ethanoigenens harbinense B 49 demonstrated higher biohydrogen yield and cellulose hydrolysis ratio compared with the mono-cultured tests. Bioaugmentation with co-cultures X 9 + B 49 efficiently improved cellulose hydrolysis and subsequent hydrogen production rates from carboxymethyl cellulose. The strain X 9 significantly hydrolyzed corn stalks pretreated with H 2SO 4, NaOH, and NH 3 soaking, and steam explosion in 10–12 h. Hydrogen was yielded in conjunction with the noted cellulose hydrolysis. The steam explosion + hydrolysis/fermentation with X 9 present the most effective method amongst the four tested pretreatments for hydrolyzing cellulose and yielding hydrogen.
ISSN:0360-3199
1879-3487
DOI:10.1016/j.ijhydene.2008.05.020