Rapid Breakdown of Phosphatidylinositol Accompanied by Accumulation of Phosphatidic Acid and Diacylglycerol in Rat Lymphocytes Stimulated by Concanavalin A
Concanavalin A stimulated the [32P]phosphate incorporation into phospholipids of rat lymph-node cells in a dose-dependent manner up to 200 µg of the lectin per ml of the lymphocyte culture. The initial step in the concanavalin A-stimulated phos-pholipid metabolism was examined by use of rat lymphocy...
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Published in | Journal of biochemistry (Tokyo) Vol. 91; no. 2; pp. 463 - 468 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.01.1982
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Subjects | |
Online Access | Get full text |
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Summary: | Concanavalin A stimulated the [32P]phosphate incorporation into phospholipids of rat lymph-node cells in a dose-dependent manner up to 200 µg of the lectin per ml of the lymphocyte culture. The initial step in the concanavalin A-stimulated phos-pholipid metabolism was examined by use of rat lymphocytes prelabeled with radioactive arachidonic acid. Concanavalin A induced a small but significant decrease, up to 10%, in the phosphatidylinositol radiolabel at 1 to 5 min after the lectin addition. A small increase, about 10%, in the diacylglycerol radiolabel and a twofold increase in the phosphatidate radiolabel were observed concomitant with the breakdown of phosphatidylinositol. The increase in the phosphatidic acid radioactivity amounted to about one-fifth of the decrease in the phosphatidylinositol radioactivity. No significant change in radiolabel was found in the other major phospholipids or in triacylglycerol. The results suggest that the breakdown of pre-existing phosphatidylinositol is the initial step in the lymphocyte phosphatidylinositol response |
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Bibliography: | ArticleID:91.2.463 istex:7E0232796396A2B6762C5E12CF71F42723B52E55 ark:/67375/HXZ-S75GX2SM-5 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-924X 1756-2651 |
DOI: | 10.1093/oxfordjournals.jbchem.a133718 |