Determination of isosorbide-5-mononitrate in human plasma by high-resolution gas chromatography

A method for the determination of isosorbide-5-mononitrate (5-ISMN) in human plasma by capillary gas chromatography with electron-capture detection was developed and applied to clinical samples. 9-Fluorenone was used as an internal standard, ethyl acetate was employed for liquid–liquid extraction. T...

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Published inJournal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 800; no. 1; pp. 271 - 274
Main Authors Pastera, J, Vysloužil, L, Květina, J
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 05.02.2004
Subjects
Chromatography, Gas
Dimethylpolysiloxanes
Fluorenes
Humans
Indicators and Reagents
Isosorbide Dinitrate - analogs & derivatives
Isosorbide Dinitrate - blood
Isosorbide Dinitrate - pharmacokinetics
Isosorbide mononitrate
Quality Control
Reference Standards
Reproducibility of Results
Silicones
Solutions
Vasodilator Agents - blood
Vasodilator Agents - pharmacokinetics
Isosorbide mononitrate
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Summary:A method for the determination of isosorbide-5-mononitrate (5-ISMN) in human plasma by capillary gas chromatography with electron-capture detection was developed and applied to clinical samples. 9-Fluorenone was used as an internal standard, ethyl acetate was employed for liquid–liquid extraction. The advantage of the extraction procedure is the possibility of a direct injection of the plasma extract, without solvent removal/reconstitution of the sample. The precision and accuracy of the method were satisfactory in the concentration range 10–1600 ng/ml. The lower limit of quantification was 10 ng/ml.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2003.10.069