Complete genome sequence of Brugmansia suaveolens mottle virus, a potyvirus from an ornamental shrub

• Published in: Archives of virology Vol. 155; no. 10; pp. 1729 - 1732
• Main Authors: Lucinda, Natalia, Inoue-Nagata, Alice K, Kitajima, Elliot W, Nagata, Tatsuya
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01.10.2010; Springer Vienna; Springer; Springer Nature B.V
• Subjects: Annotated Sequence Record; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Brazil; Cloning; Cluster Analysis; Fundamental and applied biological sciences. Psychology; Genome, Viral; Genomes; Infectious Diseases; Leaves; Medical Microbiology; Microbiology; Miscellaneous; Molecular Sequence Data; Pepper mottle virus; Phylogenetics; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - virology; Plant viruses and viroids; Potyvirus; Potyvirus - genetics; Potyvirus - isolation & purification; Proteins; RNA, Viral - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Solanaceae - virology; Virology; Brazil; Aphid Transmission; Complete Genome Sequence; Ornamental Shrub; Pepper Mottle Virus; Mottle Virus; Virus; Potyvirus; Plant pathogen; Nucleotide sequence; Potyviridae
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-010-0798-6

Brugmansia suaveolens mottle virus (BsMoV) was the first potyvirus isolated from “angel trumpet” (Brugmansia suaveolens), described in Brazil. In this study, the complete nucleotide (nt) genome sequence of BsMoV was determined, and the deduced amino acid (aa) sequence was analyzed. The BsMoV RNA genome consists of 9870 nt without a poly-A tail, encoding a putative typical potyviral polyprotein of 3090 aa. Pairwise comparisons of the complete BsMoV genome with those of the most closely related potyviruses revealed a maximum nucleotide identity of 63.7% with pepper mottle virus. These results and phylogenetic analyses based on the complete genome sequence of the most closely related potyviruses confirmed that BsMoV should be considered a member of a distinct species of the genus Potyvirus.