Evolutionary significance of different neurochemical organisation of the internal and external regions of auditory centres in the reptilian brain: an immunocytochemical and reduced NADPH-diaphorase histochemical study in turtles

• Published in: Brain research Vol. 925; no. 1; pp. 100 - 106
• Main Authors: Belekhova, M.G, Kenigfest-Rio, N.B, Vesselkin, N.P, Rio, J.-P, Repérant, J, Ward, R
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 18.01.2002; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Auditory Pathways; Auditory Pathways - cytology; Auditory system; Biochemistry, Molecular Biology; Biological Evolution; Core-belt organisation; Emys orbicularis; Enkephalin, Methionine; Enkephalin, Methionine - analysis; Evolution; Immunohistochemistry; Inferior Colliculi - cytology; Inferior Colliculus; Life Sciences; NADPH Dehydrogenase; NADPH Dehydrogenase - analysis; Neuroactive substances; Neurons; Neurons - chemistry; Neurons - enzymology; Neuropeptide Y; Neuropeptide Y - analysis; Serotonin; Serotonin - analysis; Substance P; Substance P - analysis; Testudo horsfieldi; Thalamic Nuclei; Thalamic Nuclei - cytology; Turtle; Turtles; Tyrosine 3-Monooxygenase; Tyrosine 3-Monooxygenase - analysis; Sensory systems; Turtle; Core-belt organisation; Neuroactive substances; Auditory system
• ISSN: 0006-8993; 1872-6240; 0006-8993
• DOI: 10.1016/S0006-8993(01)03255-3

An immunocytochemical and histochemical study was undertaken of the torus semicircularis and nucleus reuniens, the mesencephalic and diencephalic auditory centres, in two chelonian species, Testudo horsfieldi and Emys orbicularis. The nucleus centralis of the torus semicircularis receives few 5-HT-, TH-, substance P-, and menkephalin-immunoreactive fibres and terminals, in marked contrast to the external nucleus laminaris of the torus semicircularis, in which 5-HT-, TH-, substance P-, and menkephalin-immunoreactive elements and cell bodies show a laminar distribution. Dense NPY-positive terminal-like profiles and cell bodies were observed in both the nuclei centralis and laminaris, and many NADPH-d-positive cell bodies were observed in the cell layers of the latter. In the nucleus reuniens, the distribution of 5-HT-, TH-, substance P-, and menkephalin-immunolabelling resembles that seen in the torus semicircularis, but at a lower density. The dorsorostral regions of the nucleus reuniens, as in the nucleus centralis, is insignificantly labelled, in contrast to the ventrocaudal regions in which labelled elements abound. NPY-positive elements are uniformly distributed throughout the nucleus, but no labelled cell bodies were observed. NADPH-d-positive fibres and terminals were observed in both dorsal and ventral regions of the nucleus reuniens, but the few labelled cell bodies to be observed were located in the peripheral regions of the nucleus. These findings are discussed in terms of the evolution of the core-and-belt organisation of sensory nuclei observed in other vertebrate species.