N2/H2 plasma surface modifications of polystyrene inhibit the adhesion of multidrug resistant bacteria

• Published in: Surface & coatings technology Vol. 245; pp. 84 - 91
• Main Authors: Trentin, Danielle S., Bonatto, Fernando, Zimmer, Karine R., Ribeiro, Vanessa B., Antunes, Ana Lúcia S., Barth, Afonso L., Soares, Gabriel V., Krug, Cristano, Baumvol, Israel J.R., Macedo, Alexandre J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 25.04.2014; Elsevier
• Subjects: Bacterial adhesion; Cross-disciplinary physics: materials science; rheology; Exact sciences and technology; Materials science; Multidrug resistant bacteria; Physics; Plasma treatment; Polystyrene; Surface modification; Surface treatments; Surface modification; Bacterial adhesion; Multidrug resistant bacteria; Plasma treatment; Polystyrene; Plasma; Bacteria; Surface treatments; Adhesion
• ISSN: 0257-8972; 1879-3347
• DOI: 10.1016/j.surfcoat.2014.02.046

Adhesion is the first step in biofilm formation, a bacterial lifestyle characterized by antimicrobial resistance usually associated with implant infections. In this way, the modification of material surface by plasma processing represents an attractive strategy to understand and control bacterial–material interactions. This work challenged multidrug resistant pathogenic emerging bacteria to adhere on polystyrene submitted to radio frequency discharges. We used N2 and H2 plasma to treat polystyrene surface and we evaluated, using crystal violet assay, the ability of bacteria possessing hydrophobic or hydrophilic surfaces to adhere on the polymer, at distinct periods of shelf life. The material surface characterization was conducted using X-ray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements. It is shown that the two employed treatments, which differ in duration and power applied to the plasma, are able to inhibit up to 83% Klebsiella pneumoniae adhesion in the first 24h, without biocidal effect. Results from XPS and WCA measurements reveal that nitrogen concentration in excess of 8.8% or polar component of the surface energy above 15mJm−2 are sufficient to produce the beneficial effects sought. The correlation with biological findings indicates that the limited adhesion of bacteria possessing hydrophilic surface on plasma-treated surfaces may be explained in terms of electrostatic repulsion. Therefore, this work demonstrates that the potential application of plasma surface modification extends the possibility of controlling the adhesion of different bacterial species and genera. •N2/H2 plasma treatments produce a less bacterial adherent and non-biocidal PS material.•Hydrophilic bacteria (MRSA and KPC-producing strains) had adhesion strongly inhibited.•At least 8.8at.% N in surface, or polar surface energy of 15mJm−2, limits adhesion.•Anti-adhesion effects may be explained in terms of electrostatic repulsion.•N2/H2 plasma discharge controls adhesion by different bacterial species and genera.