Magnetic split-flow thin fractionation of magnetically susceptible particles

• Published in: Journal of Chromatography A Vol. 857; no. 1; pp. 193 - 204
• Main Authors: Fuh, C.Bor, Chen, S.Y.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.1999; Elsevier
• Subjects: Analytical biochemistry: general aspects, technics, instrumentation; Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Investigative techniques, diagnostic techniques (general aspects); Ions; Magnetics; Medical sciences; Miscellaneous. Technology; Other chromatographic methods; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Magnetic separation; Split-flow thin fractionation; Field flow fractionation; Yeast; Red blood cell; Saccharomyces; Particle separation; Silica; Magnetic susceptibility; Fungi; Separation method; Magnetic particles; Clinical biology; Ascomycetes; Microorganism; Thallophyta; Biomedical engineering
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(99)00775-X

We recently built a magnetic separation system to extend the applications of split-flow thin (SPLITT) fractionation to magnetically susceptible particles. Here, we characterize the magnetic SPLITT system using magnetically susceptible particles and ion-labeled particles. The flow axis of separation channel was orientated parallel and perpendicular to gravitational forces to exclude and include, respectively, gravitational effects on separation. Both operating modes were used to test the theory experimentally, with emphasis on the parallel mode. The magnetic susceptibilities of carrier and ion-labeled particles were varied, and various ion-labeled and unlabeled particles were studied experimentally, resulting in successful separation of labeled particles, yeasts, and cells from unlabeled ones. The minimal difference in magnetic susceptibility (Δ χ) required for complete particle separation was about 1.75×10 −5 [cgs], corresponding to about 10 9 labeling ions per particle in this study. The throughput was around 7.2×10 8 particles/h using the present setup. Magnetic SPLITT fractionation shows good potential for use in obtaining particles magnetic susceptibilities from a simple theoretical treatment.