Structural characterisation of the exopolysaccharide produced by Lactobacillus delbrückii subspecies bulgaricus rr grown in skimmed milk

• Published in: Carbohydrate research Vol. 239; pp. 209 - 226
• Main Authors: Gruter, Marijke, Leeflang, Bas R., Kuiper, Jan, Kamerling, Johannis P., Vliegenthart, Johannes F.G.
• Format: Journal Article
• Language: English
• Published: AMSTERDAM Elsevier Ltd 01.02.1993; Elsevier; Elsevier Science
• Subjects: Animals; Bacteriology; Biochemistry & Molecular Biology; Biological and medical sciences; Carbohydrate Sequence; carbohydrate structure; Chemistry; Chemistry, Applied; Chemistry, Organic; Chromatography, High Pressure Liquid; Fundamental and applied biological sciences. Psychology; galactose; glucose; Glycoproteins - chemistry; glycosidic linkages; Lactobacillus - chemistry; Lactobacillus delbrueckii; Life Sciences & Biomedicine; Magnetic Resonance Spectroscopy; Metabolism. Enzymes; Methylation; Microbiology; Milk - microbiology; Molecular Sequence Data; Oxidation-Reduction; Periodic Acid; Physical Sciences; polysaccharides; Polysaccharides, Bacterial - chemistry; Polysaccharides, Bacterial - isolation & purification; rhamnose; Science & Technology; skim milk; Spectrometry, Mass, Fast Atom Bombardment; structure; METHYLATION ANALYSIS; MONOSACCHARIDES; HOMONUCLEAR HARTMANN-HAHN; SPECTROSCOPY; POLYSACCHARIDES; CONFIGURATION; ASSIGNMENT; PROTON; CAPILLARY GLC; Microorganism culture; Bacteria; Lactobacillaceae; Biosynthesis; Chemical composition; Lactobacillus delbrueckii; Skimmilk; Polysaccharide; Structural analysis
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/0008-6215(93)84216-S

The exopolysaccharide of Lactobacillus delbrückii subsp. bulgaricus rr. isolated from skimmed milk, is a heteropolymer of d-galactopyranosyl, d-glucopyranosyl, and l-rhamnopyranosyl residues in the molar ratio 5:1:1. The structure was established by linkage analysis and 1D and 2D NMR spectroscopy of the native polysaccharide, in combination with characterisation of oligosaccharide fragments, obtained by Smith degradation and partial acid hydrolysis, using methylation analysis, EIMS, and 1D and 2D 1H NMR spectroscopy. The polysaccharide has a branched heptasaccharide repeating unit with the following structure: → 2)-[β- d-Gal p-(1 → 3)]-α- d-Gal p-(1 → 3)-β- d-Glc p-(1 → 3)-β- d-Glc p-(1 → 4)]-β- d-Gal p-(1 → 4)-[α- l-Rha p-(1 → 3)]-α- d-Gal p-(1 →.