Removal of monomer delignification products by laccase from Trametes versicolor
► Effect of laccase on lignin derived phenolic monomers in wheat straw hydrolyzates. ► Identification of three reaction groups of phenolic monomers towards laccase (LRG). ► Compounds of LRG-B and -C important in respect to detoxification for fermentation. ► Different results comparing phenolic monom...
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Published in | Bioresource technology Vol. 104; pp. 298 - 304 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.01.2012
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Subjects | |
Online Access | Get full text |
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Summary: | ► Effect of laccase on lignin derived phenolic monomers in wheat straw hydrolyzates. ► Identification of three reaction groups of phenolic monomers towards laccase (LRG). ► Compounds of LRG-B and -C important in respect to detoxification for fermentation. ► Different results comparing phenolic monomer analytic and total phenol content. ► HS-SPME/GC–MS powerful method analyzing volatile compounds in hydrolyzates.
The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined. Beside the total phenol content derived by Folin–Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC–MS. A notable decrease of the phenols was achieved using 0.2 and 0.5U/mL laccase whilst higher dosage showed no improvement. Nearly all kind of monomer phenolic compounds identified in the LHW-S were found to be removed after 24h. However, acetophenone and 4-hydroxybenzaldehyde (HBA) were obviously not affected by laccase. Summarizing, three laccase reaction groups (LRG) of phenolic monomers could be classified: immediate removal (LRG-A), degradation after 1day (LRG-B), no effect of laccase (LRG-C). Additionally, HS-SPME/GC was found to be a powerful tool to study the reaction of laccase and phenolic monomers in complex lignocellulose derived solutions. |
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Bibliography: | http://dx.doi.org/10.1016/j.biortech.2011.11.080 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0960-8524 1873-2976 |
DOI: | 10.1016/j.biortech.2011.11.080 |