Molecular variation of hop mosaic virus isolates

• Published in: Archives of virology Vol. 155; no. 10; pp. 1721 - 1724
• Main Authors: Poke, Fiona S, Crowle, Damian R, Whittock, Simon P, Wilson, Calum R
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01.10.2010; Springer Vienna; Springer; Springer Nature B.V
• Subjects: Amino acids; Annotated Sequence Record; Australia; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Capsid Proteins - genetics; Carlavirus; Carlavirus - classification; Carlavirus - genetics; Carlavirus - isolation & purification; Cluster Analysis; Cultivars; Czech Republic; Fundamental and applied biological sciences. Psychology; Genomes; Hop mosaic virus; Humulus - virology; Infections; Infectious Diseases; Japan; Medical Microbiology; Microbiology; Miscellaneous; Molecular Sequence Data; Open Reading Frames; Phylogenetics; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - virology; Plant viruses and viroids; Polymorphism, Genetic; Proteins; RNA, Viral - genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; South Africa; United States; Virology; South Africa; Czech Republic; Australia; United States; Japan; United States > US; Tasmania Australia; Amino Acid Sequence Data; Coat Protein; Amino Acid Variability; Triple Gene Block; Coat Protein Amino Acid; Carlavirus; Virus; Isolate; Plant pathogen; Hop mosaic virus; Flexiviridae
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-010-0780-3

Hop mosaic virus (HpMV), a member of the genus Carlavirus, is importance to hop production worldwide. We identified variation in nucleic and amino acid sequences among 23 HpMV isolates from Australia, the USA, the Czech Republic, South Africa and Japan using a 1,455-bp fragment covering the 3′ end of the virus genome including ORFs 4, 5 and 6. Three clusters of two or more isolates were identified in phylogenies of the total nucleotide sequence and the coat protein (ORF5) amino acid sequence. Two of these clusters combined in analyses of ORF4 and ORF6 amino acid sequences. Isolates from within and outside of Australia were found in each cluster, indicating that sequence variation was not associated with geographic source. Monitoring of HpMV variants in the field and evaluation of the impact of variants on vector association, rate of spread, and hop yield and quality can now be undertaken.