Cytoprotective role of Solanum nigrum against gentamicin-induced kidney cell (Vero cells) damage in vitro

• Published in: Fitoterapia Vol. 72; no. 5; pp. 481 - 486
• Main Authors: Prashanth Kumar, V., Shashidhara, S., Kumar, M.M., Sridhara, B.Y.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.2001; Elsevier
• Subjects: Animals; Biological and medical sciences; Cercopithecus aethiops; Cytoprotective activity; Free Radical Scavengers - pharmacology; General pharmacology; Gentamicins; Humans; Kidney - metabolism; Medical sciences; Mitochondria - drug effects; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Extracts - pharmacology; Plants, Medicinal; Protective Agents - pharmacology; Solanaceae; Solanum nigrum; Trypan Blue; Vero cells; Vero Cells - drug effects; Asia; India; Cytoprotective activity; Solanum nigrum; Vero cells; Toxicity; Gentamicin; Monkey; Cytotoxicity; Free radical; Kidney; Medicinal plant; Prevention; Folk medicine; Dicotyledones; Angiospermae; Established cell line; Primates; Solanaceae; Kidney disease; Urinary system disease; Pharmacognosy; Antioxidant; In vitro; Biological activity; Vertebrata; Antibiotic; Trapping; Mammalia; Cytoprotector; Aminoglycoside; Animal; Plant origin; Spermatophyta; Antibacterial agent; Hydroxyl
• ISSN: 0367-326X
• DOI: 10.1016/S0367-326X(01)00266-0

The 50% ethanol extract of the whole plant of Solanum nigrum was tested in vitro for its cytoprotection against gentamicin-induced toxicity on Vero cells. Cytotoxicity was significantly inhibited as assessed by the Trypan blue exclusion assay and mitochondrial dehydrogenase activity (MTT) assay. The test extract also exhibited significant hydroxyl radical scavenging potential, thus suggesting its probable mechanism of cytoprotection.