Myricetin, a naturally occurring flavonoid, prevents 2-deoxy- d-ribose induced dysfunction and oxidative damage in osteoblastic MC3T3-E1 cells

• Published in: European journal of pharmacology Vol. 591; no. 1; pp. 1 - 6
• Main Authors: Lee, Kyung-Hee, Choi, Eun-Mi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 04.09.2008; Elsevier
• Subjects: 2-Deoxy- d-ribose; Alkaline Phosphatase - drug effects; Alkaline Phosphatase - metabolism; Animals; Biological and medical sciences; Bone Diseases - drug therapy; Bone Diseases - etiology; Calcium - metabolism; Cell Line; Cell Survival - drug effects; Collagen - drug effects; Collagen - metabolism; Deoxyribose - toxicity; Diabetes Complications - physiopathology; Diabetes Complications - prevention & control; Dietary Supplements; Flavonoids - pharmacology; Glucose toxicity; Malondialdehyde - metabolism; Medical sciences; Mice; Myricetin; Osteoblasts - drug effects; Osteoblasts - metabolism; Oxidative stress; Oxidative Stress - drug effects; Pharmacology. Drug treatments; Protein Carbonylation - drug effects; Oxidative stress; Glucose toxicity; 2-Deoxy- d-ribose; Myricetin; Polyphenol; Toxicity; Phenols; Glucose; Lesion; Flavonoid; In vitro; 2-Deoxy-D-ribose,Glucose toxicity,Oxidative stress,Myricetin
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2008.06.004

Myricetin, a naturally occurring flavonoid, was investigated to determine whether it could protect osteoblasts from 2-deoxy- d-ribose induced dysfunction and oxidative damage in the MC3T3-E1 cells. MC3T3-E1 cells were incubated with 2-deoxy- d-ribose and/or myricetin, and markers of osteoblast function and oxidative damage were examined. Compared with control incubation, 2-deoxy- d-ribose significantly ( P < 0.05) inhibited alkaline phosphatase (ALP) activity, collagen content, and calcium deposition at the concentration of 20 mM. Cellular malondialdehyde (MDA), protein carbonyl, and advanced oxidation protein products contents were significantly ( P < 0.05) increased in the presence of 2-deoxy- d-ribose (20 mM). Myricetin significantly ( P < 0.05) increased cell survival, ALP activity, collagen, osteocalcin, osteoprotegerin, and calcium deposition and decreased MDA, protein carbonyl, and advanced oxidation protein products contents of osteoblastic MC3T3-E1 cells in the presence of 20 mM 2-deoxy- d-ribose. These results demonstrate that myricetin attenuates 2-deoxy- d-ribose induced damage, suggesting that myricetin may be a useful dietary supplement for minimizing oxidative injury in diabetes related bone diseases.