Regio- and stereo-selective hydroxylation of abietic acid derivatives by Mucor circinelloides and Mortierella isabellina

• Published in: Biotechnology letters Vol. 27; no. 17; pp. 1305 - 1310
• Main Authors: MITSUKURA, Koichi, IMOTO, Takeshi, NAGAOKA, Hirokazu, YOSHIDA, Toyokazu, NAEASAWA, Toru
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.09.2005; Springer Nature B.V
• Subjects: Acids; Bacteria; Biological and medical sciences; Biotechnology; Cells; Diterpenes, Abietane - analysis; Diterpenes, Abietane - metabolism; Fundamental and applied biological sciences. Psychology; Hydroxylation; Mortierella; Mortierella - metabolism; Mortierella isabellina; Mucor - metabolism; Mucor circinelloides; Stereoisomerism; abietic acid derivatives; Hydroxylation; Regioselectivity; Phycomycetes; stereo-selective; Mortierella; Fungi; Stereoselectivity; Acids; microbial hydroxylation; Mucor circinelloides; Microorganism; regio-selective; Thallophyta
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-005-3224-5

Mucor circinelloides and Mortierella isabellina hydroxylated dehydroabietic acid (DehA). DehA was converted regio- and stereo-selectively by whole cells of Mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mM from 14.7 mM DehA) after 72 h in the cultivation medium containing 3% (v/v) Tween 80. With cells of Ma. isabellina, under the same conditions, 20.5 mM (6.5 g l(-1)) 2-hydroxydehydroabietic acid (alpha/beta=81/19) was formed from 26.4 mM DehA.