New cytokine dressings. II. Stimulation of oxidative burst in leucocytes in vitro and reduction of viable bacteria within an infected wound

• Published in: International journal of pharmaceutics Vol. 184; no. 2; pp. 179 - 187
• Main Authors: Grzybowski, Jacek, Janiak, Marek K, Oidak, Ewa, Lasocki, Krzysztof, Wrembel-Wargocka, Jolanta, Cheda, Aneta, Antos-Bielska, Malgorzata, Pojda, Zygmunt
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20.07.1999; Elsevier
• Subjects: Animals; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacteria - drug effects; Bandages; Biological and medical sciences; Cytokine dressings; Cytokines - administration & dosage; Cytokines - pharmacology; Drug Delivery Systems; Epidermal Growth Factor - administration & dosage; Epidermal Growth Factor - pharmacology; Flow Cytometry; General pharmacology; Granulocyte Colony-Stimulating Factor - administration & dosage; Granulocyte Colony-Stimulating Factor - pharmacology; Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology; Humans; Leukocyte Count; Leukocytes - drug effects; Leukocytes - metabolism; Medical sciences; Mice; Mice, Inbred C3H; Murine model of infected wound; Oxidative burst; Phagocytic activity; Phagocytosis - drug effects; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Recombinant Proteins - administration & dosage; Recombinant Proteins - pharmacology; Respiratory Burst - drug effects; rhG-CSF; rhGM-CSF; Topical cytokine-delivery system; Wound Healing - drug effects; Wounds and Injuries - microbiology; Cytokine dressings; rhG-CSF; Oxidative burst; Phagocytic activity; rhGM-CSF; Topical cytokine-delivery system; Murine model of infected wound; Pseudomonadales; Skin disease; Wound; Dressing; Granulocyte colony stimulating factor; Bacteria; Pseudomonadaceae; Pseudomonas aeruginosa; Recombinant protein; Delivery system; Pharmaceutical technology; Rodentia; Cytokine; Biological activity; Infection; Vertebrata; Mammalia; Mouse; Animal; Collagen; Polyurethane; Dosage form; Antibacterial agent; Granulocyte macrophage colony stimulating factor; Phagocytosis; Topical administration
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/S0378-5173(99)00064-2

Recently, we have developed the new dressings containing rhG-CSF or rhGM-CSF. In the present study we investigated either in vitro or in vivo biological activity of the dressings. Human whole blood samples were incubated with extracts from the collagen- or polyurethane-based dressings containing rhG-CSF or/and rhGM-CSF and phagocytic and oxidative metabolic activities were quantitated using Phagotest or Bursttest kits. The results indicate that both the number of phagocyting cells and the intensity of phagocytosis per cell, as well as the level of the oxidative burst in particular, were stimulated by one or both of the cytokines extracted. Next, the experimental skin wounds in mice were infected with 10 7 CFU of Pseudomonas aeruginosa strain ATCC 27853 and treated locally with the rhG-CSF-containing dressing. The analysis of the biopsies taken from the wounds indicated that in mice treated with the cytokine-containing dressing on the third day the log of CFU per biopsy was 5.0 vs. 6.2 in the control ( P<0.001), and on the 8th day was lower than 4 vs. 5.4 in control ( P<0.0001). Our findings clearly suggest that the newly designed dressings containing the incorporated CSFs can be used as effective topical cytokine-delivery system in the treatment of bacterial infections in wounds.