Increased Na+/Ca2+ exchanger activity promotes resistance to excitotoxicity in cortical neurons of the ground squirrel (a Hibernator)

• Published in: PloS one Vol. 9; no. 11; p. e113594
• Main Authors: Zhao, Juan-Juan, Gao, Shan, Jing, Jun-Zhan, Zhu, Ming-Yue, Zhou, Chen, Chai, Zhen
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.11.2014; Public Library of Science (PLoS)
• Subjects: Adaptation, Physiological; Animals; Biology and Life Sciences; Biotechnology; Body temperature; Brain; Brain damage; Brain injury; Calcium; Calcium (intracellular); Calcium - metabolism; Calcium content; Calcium homeostasis; Calcium influx; Cell survival; Cells, Cultured; Cerebral Cortex - cytology; Cerebral Cortex - drug effects; Cerebral Cortex - injuries; Endoplasmic reticulum; Excitotoxicity; Glucose; Glutamate receptors; Glutamic Acid - toxicity; Glutamic acid receptors; Ground squirrels; Hibernation; Homeostasis; Ischemia; Laboratory animals; Life sciences; Mammals; Medicine and Health Sciences; Membrane Potential, Mitochondrial - drug effects; Metabolism; N-Methyl-D-aspartic acid; Na+/Ca2+ exchanger; Na+/Ca2+-exchanging ATPase; NCX1 protein; Neurons; Neurons - drug effects; Neurons - physiology; Neurotoxicity; Perfusion; Plasma; Rats; Rats, Sprague-Dawley; Rodents; Sciuridae; Sodium - metabolism; Sodium-Calcium Exchanger - physiology; Spermophilus dauricus; Squirrels; Stroke; Toxicity; Beijing China; United States > US; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0113594

Ground squirrel, a hibernating mammalian species, is more resistant to ischemic brain stress than rat. Gaining insight into the adaptive mechanisms of ground squirrels may help us design treatment strategies to reduce brain damage in patients suffering ischemic stroke. To understand the anti-stress mechanisms in ground squirrel neurons, we studied glutamate toxicity in primary cultured neurons of the Daurian ground squirrel (Spermophilus dauricus). At the neuronal level, for the first time, we found that ground squirrel was more resistant to glutamate excitotoxicity than rat. Mechanistically, ground squirrel neurons displayed a similar calcium influx to the rat neurons in response to glutamate or N-methyl-D-aspartate (NMDA) perfusion. However, the rate of calcium removal in ground squirrel neurons was markedly faster than in rat neurons. This allows ground squirrel neurons to maintain lower level of intracellular calcium concentration ([Ca2+]i) upon glutamate insult. Moreover, we found that Na+/Ca2+ exchanger (NCX) activity was higher in ground squirrel neurons than in rat neurons. We also proved that overexpression of ground squirrel NCX2, rather than NCX1 or NCX3, in rat neurons promoted neuron survival against glutamate toxicity. Taken together, our results indicate that ground squirrel neurons are better at maintaining calcium homeostasis than rat neurons and this is likely achieved through the activity of ground squirrel NCX2. Our findings not only reveal an adaptive mechanism of mammalian hibernators at the cellular level, but also suggest that NCX2 of ground squirrel may have therapeutic value for suppressing brain ischemic damage.