CRISAMICIN A, A NEW ANTIBIOTIC FROM MICROMONOSPORA I. TAXONOMY OF THE PRODUCING STRAIN, FERMENTATION, ISOLATION, PHYSICO-CHEMICAL CHARACTERIZATION AND ANTIMICROBIAL PROPERTIES

• Published in: Journal of antibiotics Vol. 39; no. 3; pp. 335 - 344
• Main Authors: NELSON, RICHARD A., POPE, JOSEPH A., Jr, LUEDEMANN, GEORGE M., MCDANIEL, LLOYD E., SCHAFFNER, CARL P.
• Format: Journal Article
• Language: English
• Published: Tokyo JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 1986; Japan Antibiotics Research Association
• Subjects: Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - isolation & purification; Antibiotics; Biological and medical sciences; Biotechnology; Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Fundamental and applied biological sciences. Psychology; General pharmacology; Health. Pharmaceutical industry; Industrial applications and implications. Economical aspects; Medical sciences; Naphthoquinones - isolation & purification; Pharmacology. Drug treatments; Physicochemical properties. Structure-activity relationships; Production of active biomolecules; Actinomycetales; Antibiotic; Taxonomy; Micromonosporaceae; Production; Bacteria; Isolation; Actinomycetes; Micromonospora purpureochromogenes; Antibacterial agent; Fermentation; Physicochemical properties
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.39.335

A microorganism, designated as RV-79-9-101 and now identified as Micromonospora purpureochromogenes subsp. halotolerans, isolated from a mud sample in the Philippines, has been shown to produce a complex of antibiotics called crisamicins. Thin-layer chromatography and bioautography, employing solvent extracts of whole fermentation broths, revealed a minimum of five antimicrobial components. The major biologically-active component of the antibiotic complex, crisamicin A, was obtained in pure form after preparative silica gel column chromatography followed by crystallization. Based on physico-chemical data crisamicin A has been identified as a novel member of the isochromanequinone group of antibiotics. It exhibits excellent in vitro activity against Gram-positive bacteria but little or no activity towards Gram-negative bacteria or fungi.