Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold-Coated Membranes

A method for the one‐step purification of high‐complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full‐length peptides covalently couple to the receptor membrane via an...

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Published inAdvanced materials (Weinheim) Vol. 25; no. 11; pp. 1598 - 1602
Main Authors Schirwitz, Christopher, Loeffler, Felix F., Felgenhauer, Thomas, Stadler, Volker, Nesterov-Mueller, Alexander, Dahint, Reiner, Breitling, Frank, Bischoff, F. Ralf
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 20.03.2013
WILEY‐VCH Verlag
Subjects
Arrays
Covalence
Cysteine
high-complexity
high-density
Joining
laser printers
Membranes
microchips
peptide microarrays
Peptides
Purification
Receptors
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Summary:A method for the one‐step purification of high‐complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full‐length peptides covalently couple to the receptor membrane via an N‐terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm2 is demonstrated.
Bibliography:istex:E1F785F1D196D73B49B759ACE4B13425B28CA4F1
ArticleID:ADMA201203853
ark:/67375/WNG-188500Z4-2
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0935-9648
1521-4095
DOI:10.1002/adma.201203853