Definitive chemical evidence for the constitutive ability of Candida albicans serotype A strains to synthesize β-1,2 linked oligomannosides containing up to 14 mannose residues

• Published in: FEBS letters Vol. 416; no. 2; pp. 203 - 206
• Main Authors: Trinel, P.A., Lepage, G., Jouault, T., Strecker, G., Poulain, D.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 20.10.1997
• Subjects: Candida albicans; Candida albicans - classification; Candida albicans - metabolism; Carbohydrate Sequence; Chromatography, Gel; Chromatography, Thin Layer; Degree of polymerization; Mannan; Mannans - biosynthesis; Mannans - chemistry; Mannans - isolation & purification; Molecular Sequence Data; NMR analysis; Nuclear Magnetic Resonance, Biomolecular; Oligosaccharides - biosynthesis; Oligosaccharides - chemistry; Oligosaccharides - pharmacology; Phosphopeptides - chemistry; Phosphopeptides - isolation & purification; Serotype A; Serotyping; Tumor Necrosis Factor-alpha - biosynthesis; β-1,2 linked oligomannoside; Candida albicans; Serotype A; Degree of polymerization; Mannan; β-1,2 linked oligomannoside; NMR analysis
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(97)01205-2

We have previously reported the presence of phosphate bound β-1,2 linked oligomannosides with unusually high degrees of polymerization (DP>7) in the mannan of Candida albicans strain VW32. To confirm this observation, we have prepared these oligomannosides from the mannan of C. albicans strain NIH A 207. Gel filtration chromatography and TLC analysis revealed DP up to 14. For both strains, NMR analysis confirmed the exclusive presence of β-1,2 linkages in the pools of oligomannosides with a DP higher than 6 which presented an average DP of 10.6 (VW32) and 10.4 (NIH A 207). These results are important to consider in relation with the ability of these C. albicans derived oligomannosides to trigger TNFα synthesis according to their DP.