Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro

• Published in: Asian Pacific journal of tropical biomedicine Vol. 9; no. 2; pp. 66 - 72
• Main Authors: Dutra, Jean, De Oliveira, Judá, Dos Santos, Vanessa, Pereira, Paula, Ferreira, Jean, Batitucci, Maria Do
• Format: Journal Article
• Language: English
• Published: Medknow Publications and Media Pvt. Ltd 01.02.2019; Laboratório de Genética Vegetal e Toxicológica, Departamento de Ciências Biológicas, Universidade Federal do Espírito Santo, 29075-910, Vitória, Espírito Santo, Brazil%Laboratório de Biologia Molecular e Express?o Gênica, Departamento de Ciências Biológicas, Universidade Federal de Alagoas, 57309-005,Arapiraca, Alagoas, Brazil; Wolters Kluwer Medknow Publications
• Subjects: abts; Aluminum compounds; Analysis; Antioxidants (Nutrients); dpph; fe2+ ion chelating activity; flavonoid content; Flavonoids; Isoflavones; mandacaru; mtt assay; Sarcoma; sarcoma 180 cells; Seeds; Sarcoma 180 cells; DPPH; Mandacaru; Fe2+ ion chelating activity; Flavonoid content; MTT assay; ABTS
• ISSN: 2221-1691; 2588-9222
• DOI: 10.4103/2221-1691.250857

Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru (C. jamacaru) (mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl3 colorimetric method, antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity and Fe2+ ion chelating activity, and in vitro antiproliferative effects (sarcoma 180 cells) by 3-(4,5- dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Results: The extract of C. jamacaru cladodes at the fruiting stage showed higher flavonoid content compared to the other extracts. Seed extracts showed the highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays, and the extract of cladodes at vegetative stage showed better antioxidant activity in Fe2+ ion chelating activity. The extract of fruiting cladodes promoted higher antiproliferative effects compared to the other extracts. Conclusions: These findings suggest that fruiting increases the content of flavonoids and antiproliferative effects of C. jamacaru cladodes. Data reinforce the potential use of C. jamacaru cladodes and fruits as natural antioxidants and potent anticancer agent.