Diffusion systems for evaluation of biomineralization

A variety of in vitro study methods have been used to elucidate the roles of matrix molecules in biomineralization processes. Among these, gel diffusion-precipitation studies have proved to be an effective tool. This methodology is uniquely capable of characterizing the effects of matrix molecules o...

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Bibliographic Details
Published inCalcified tissue international Vol. 75; no. 6; pp. 494 - 501
Main Authors Silverman, L, Boskey, A L
Format Journal Article
LanguageEnglish
Published United States Springer Nature B.V 01.12.2004
Subjects
Agar - metabolism
Calcification, Physiologic
Calcium - metabolism
Cell-Free System
Chemical Precipitation
Diffusion
Durapatite - metabolism
Extracellular Matrix - metabolism
Gelatin - metabolism
Gels
Laboratory Investigations
Phosphates - metabolism
Sepharose - metabolism
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Summary:A variety of in vitro study methods have been used to elucidate the roles of matrix molecules in biomineralization processes. Among these, gel diffusion-precipitation studies have proved to be an effective tool. This methodology is uniquely capable of characterizing the effects of matrix molecules on mineralization while only using very small quantities of material. Furthermore, gel methods have been extended for use as a mineralization assay system to characterize modified matrix molecules and synthetic analogues. Here we discuss the advantages and limitations of gelatin, agar, agarose, and other systems for studying the mechanisms of biomineralization.
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ISSN:0171-967X
1432-0827
DOI:10.1007/s00223-004-0019-y