Quantitative determination of paroxetine in plasma by high-performance liquid chromatography and ultraviolet detection

• Published in: Journal of chromatography. B, Biomedical sciences and applications Vol. 693; no. 1; pp. 147 - 151
• Main Authors: Foglia, John P, Sorisio, Denise, Kirshner, Margaret, Pollock, Bruce G
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 23.05.1997; Elsevier Science
• Subjects: Aged; Analysis; Antidepressive Agents, Second-Generation - blood; Antidepressive Agents, Second-Generation - therapeutic use; Biological and medical sciences; Chromatography, High Pressure Liquid; Double-Blind Method; Drug Monitoring; General pharmacology; Humans; Medical sciences; Paroxetine; Paroxetine - blood; Paroxetine - therapeutic use; Pharmacology. Drug treatments; Reproducibility of Results; Serotonin Uptake Inhibitors - blood; Serotonin Uptake Inhibitors - therapeutic use; Spectrophotometry, Ultraviolet; Paroxetine; Human; Biological fluid; Ultraviolet detector; Serotonin; Piperidine derivatives; HPLC chromatography; Antidepressant agent; Reuptake inhibitor; Quantitative analysis; Blood plasma
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(97)00010-8

An accurate, reliable procedure was developed for kinetic and therapeutic monitoring of paroxetine in human plasma. Steady-state plasma levels of paroxetine were measured for 18 geriatric patients (mean age 75) in a double-blinded study. Paroxetine doses ranged from 10 to 40 mg/day. The assay was suitable for patients on concurrent medications, and a small sample volume (1 ml) of patient plasma was used with sufficient sensitivity and specificity. After extraction and separation on a Beckman, Ultrasphere 5-μm C 18 column (150×2 mm I.D.), the recovery (mean±S.D.) for paroxetine was determined to be 86.5±5.2%. The limit of quantitation for paroxetine in this assay was 5 ng/ml. Inter-assay reproducibility (C.V.) for the patient samples and quality controls ranged from 3.7 to 7.6%.