Enzymatic hydrolysis of the conjugate of vitamin D and related compounds

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 15; no. 9; pp. 1207 - 1214
• Main Authors: Shimada, Kazutake, Mitamura, Kuniko, Saito, Kyoko, Ohtake, Yukari, Nakatani, Ito
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.1997; Elsevier Science
• Subjects: Analysis; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Enzymatic hydrolysis; General pharmacology; Glucuronidase - chemistry; Hydrolysis; Medical sciences; Monoconjugate; Pharmacology. Drug treatments; Pro-form; Reversed-phase high-performance liquid chromatography; Substrate Specificity; Sulfatases - chemistry; Vitamin D; Vitamin D - analogs & derivatives; Vitamin D - chemistry; Reversed-phase high-performance liquid chromatography; Monoconjugate; Vitamin D; Pro-form; Enzymatic hydrolysis; Substrate specificity; β-Glucuronidase; Enzyme; HPLC chromatography; Esterases; Glucuroconjugate; Hydrolysis; Separation method; Reversed phase chromatography; Ultraviolet detector; Glycosidases; Cholecalciferol(25-hydroxy); Organic sulfate; Arylsulfatase; Hydrolases; Sulfuric ester hydrolases; Conjugated compound; O-Glycosidases
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(96)01983-8

The monoglucuronides of vitamin D, 25-hydroxyvitamin D and the corresponding pro-forms were subjected to enzymatic hydrolysis using β-glucuronidase, and substrate specificities were found in the examined enzymes originating from different sources, which were determined using reversed-phase high-performance liquid chromatography with UV detection. The enzymatic hydrolysis of the corresponding monosulfates was also examined using the same system.