Exogenous Polyunsaturated Fatty Acids Impact Membrane Remodeling and Affect Virulence Phenotypes among Pathogenic Vibrio Species

• Published in: Applied and environmental microbiology Vol. 83; no. 22; p. 1
• Main Authors: Moravec, Anna R, Siv, Andrew W, Hobby, Chelsea R, Lindsay, Emily N, Norbash, Layla V, Shults, Daniel J, Symes, Steven J K, Giles, David K
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 15.11.2017
• Subjects: Aquatic environment; Bacteria; Biofilms; Cell Membrane - chemistry; Cell Membrane - metabolism; Cell membranes; Chromatography; Chromatography, Thin Layer; Exposure; Fatty acids; Fatty Acids, Unsaturated - chemistry; Fatty Acids, Unsaturated - metabolism; Foodborne diseases; Foodborne pathogens; Health risks; Humans; Hydrophobicity; Ingestion; Liquid chromatography; Machinery and equipment; Mass Spectrometry; Mass spectroscopy; Membrane permeability; Membranes; Pathogens; Permeability; Phospholipids; Phospholipids - chemistry; Phospholipids - metabolism; Physiology; Polyunsaturated fatty acids; Seafood; Skin; Thin layer chromatography; Vibrio; Vibrio cholerae - chemistry; Vibrio cholerae - genetics; Vibrio cholerae - metabolism; Vibrio cholerae - pathogenicity; Vibrio Infections - microbiology; Vibrio parahaemolyticus - chemistry; Vibrio parahaemolyticus - genetics; Vibrio parahaemolyticus - metabolism; Vibrio parahaemolyticus - pathogenicity; Vibrio vulnificus - chemistry; Vibrio vulnificus - genetics; Vibrio vulnificus - metabolism; Vibrio vulnificus - pathogenicity; Virulence; Water pollution; Waterborne diseases; Wounds; fatty acids; Vibrio cholerae; Vibrio vulnificus; Vibrio parahaemolyticus; motility; phospholipids; biofilms; Vibrio
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.01415-17

The pathogenic species ( , , and ) represent a constant threat to human health, causing foodborne and skin wound infections as a result of ingestion of or exposure to contaminated water and seafood. Recent studies have highlighted 's ability to acquire fatty acids from environmental sources and assimilate them into cell membranes. The possession and conservation of such machinery provokes consideration of fatty acids as important factors in the pathogenic lifestyle of species. The findings here link exogenous fatty acid exposure to changes in bacterial membrane phospholipid structure, permeability, phenotypes associated with virulence, and consequent stress responses that may impact survival and persistence of pathogenic species. Polyunsaturated fatty acids (PUFAs) (ranging in carbon length and unsaturation) supplied in growth medium were assimilated into bacterial phospholipids, as determined by thin-layer chromatography and liquid chromatography-mass spectrometry. The incorporation of fatty acids variably affected membrane permeability, as judged by uptake of the hydrophobic compound crystal violet. For each species, certain fatty acids were identified as affecting resistance to antimicrobial peptide treatment. Significant fluctuations were observed with regard to both motility and biofilm formation following growth in the presence of individual PUFAs. Our results illustrate the important and complex roles of exogenous fatty acids in the membrane physiology and virulence of a bacterial genus that inhabits aquatic and host environments containing an abundance of diverse fatty acids. Bacterial responses to fatty acids include, but are not limited to, degradation for metabolic gain, modification of membrane lipids, alteration of protein function, and regulation of gene expression. species exhibit significant diversity with regard to the machinery known to participate in the uptake and incorporation of fatty acids into their membranes. Both aquatic and host niches occupied by are rife with various free fatty acids and fatty acid-containing lipids. The roles of fatty acids in the environmental survival and pathogenesis of bacteria have begun to emerge and are expected to expand significantly. The current study demonstrates the responsiveness of , , and to exogenous PUFAs. In addition to phospholipid remodeling, PUFA assimilation impacts membrane permeability, motility, biofilm formation, and resistance to polymyxin B.