Five Rice Seed-Specific NF-YC Genes Redundantly Regulate Grain Quality and Seed Germination via Interfering Gibberellin Pathway

NF-YCs are important transcription factors with diverse functions in the plant kingdoms including seed development. NF-YC8, 9, 10, 11 and 12 are close homologs with similar seed-specific expression patterns. Despite the fact that some of the NF-YCs are functionally known; their biological roles have...

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Published inInternational journal of molecular sciences Vol. 23; no. 15; p. 8382
Main Authors Xu, Huayu, Li, Shufan, Kazeem, Bello Babatunde, Ajadi, Abolore Adijat, Luo, Jinjin, Yin, Man, Liu, Xinyong, Chen, Lijuan, Ying, Jiezheng, Tong, Xiaohong, Wang, Yifeng, Niu, Baixiao, Chen, Chen, Zeng, Xiaoshan, Zhang, Jian
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.08.2022
MDPI
Subjects
Abscisic acid
Genes
Germination
Gibberellins
Grain
Homology
Mutation
NF-YCs
Phenotypes
Proteins
Quality control
Rice
rice (Oryza sativa L.)
Seed germination
Seeds
Transcription factors
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Summary:NF-YCs are important transcription factors with diverse functions in the plant kingdoms including seed development. NF-YC8, 9, 10, 11 and 12 are close homologs with similar seed-specific expression patterns. Despite the fact that some of the NF-YCs are functionally known; their biological roles have not been systematically explored yet, given the potential functional redundancy. In this study, we generated pentuple mutant pnfyc of NF-YC8-12 and revealed their functions in the regulation of grain quality and seed germination. pnfyc grains displayed significantly more chalkiness with abnormal starch granule packaging. pnfyc seed germination and post-germination growth are much slower than the wild-type NIP, largely owing to the GA-deficiency as exogenous GA was able to fully recover the germination phenotype. The RNA-seq experiment identified a total of 469 differentially expressed genes, and several GA-, ABA- and grain quality control-related genes might be transcriptionally regulated by the five NF-YCs, as revealed by qRT-PCR analysis. The results demonstrated the redundant functions of NF-YC8-12 in regulating GA pathways that underpin rice grain quality and seed germination, and shed a novel light on the functions of the seed-specific NF-YCs.
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These authors contributed equally to this work.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23158382