The role of MLC901 in reducing VEGF as a vascular permeability marker in rats with spinal cord injury

• Published in: Annals of medicine and surgery Vol. 75; p. 103344
• Main Authors: Wahyudi, Islam, Andi Asadul, Hatta, Mochammad, Adhimarta, Willy, Faris, Muhammad, Mustamir, Nasrullah, Bukhari, Agussalim, Prihantono, Kaelan, Cahyono, Hendarto, Joko, Imran, Naufal Hilmy, Rosyidi, Rohadi Muhammad
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2022; Elsevier
• Subjects: Experimental Research; Increased vascular permeability; MLC 901; Regulation of VEGF level; Spinal cord injury; Tissue edema; MLC 901; Increased vascular permeability; Tissue edema; Spinal cord injury; Regulation of VEGF level
• ISSN: 2049-0801; 2049-0801
• DOI: 10.1016/j.amsu.2022.103344

Damaged neural tissue caused by SCI could induce vascular endothelial growth factor (VEGF) that can worsen the condition in the late phase by increasing vascular permeability, thus inducing tissue oedema, which can worsen the infarction. MLC 901 has been widely used in Asia for stroke patients because its mechanism is known to down-regulate VEGF levels in ischemic tissue. Ten Sprague-Dawley rats were used in this experiment. To create a severe spinal cord injury in animal models. The animals were then randomly divided into two groups. MLC 901 was given to the first group, which was the intervention group, and placebo to the second group, which was the control group. This study showed a decrease in the mean VEGF mRNA expression in the group given MLC 901 compared to the control group, which had a very high mean VEGF mRNA expression starting after 1 h of administration of MLC 901 until day 14 after spinal cord injury. In addition, there was a decrease in VEGF levels in the MLC 901 group compared to the control group from 3 h after spinal cord injury (1 h after MLC 901 administration) to 14 days after spinal cord injury. It can be concluded that administration of MLC 901 can reduce vascular permeability, one of the mechanisms that is thought to occur is to reduce VEGF levels. MLC 901 also maintains the neuroprotective effect provided by VEGF by maintaining this level above the basal level until day 14. •Experimental study on mice subjects to with spinal cord injury.•This Research to Evaluate of VEGF level in rats with spinal cord injury and also in VEGF gene mRNA expression between treatment (MLC901) group and control group in rats with spinal cord injury.•MLC 901 also maintains the neuroprotective effect provided by VEGF by maintaining this level above the basal level until day 14.