Awa1p on the cell surface of sake yeast inhibits biofilm formation and the co-aggregation between sake yeasts and Lactobacillus plantarum ML11-11

• Published in: Journal of bioscience and bioengineering Vol. 119; no. 5; pp. 532 - 537
• Main Authors: Hirayama, Satoru, Shimizu, Masashi, Tsuchiya, Noriko, Furukawa, Soichi, Watanabe, Daisuke, Shimoi, Hitoshi, Takagi, Hiroshi, Ogihara, Hirokazu, Morinaga, Yasushi
• Format: Journal Article
• Language: English
• Published: Japan Elsevier B.V 01.05.2015
• Subjects: Alcoholic Beverages - microbiology; Awa1p; Biofilms - growth & development; Cell Adhesion; Cell Aggregation; Cell Wall - metabolism; Co-aggregation; Glycosylphosphatidylinositols - genetics; Glycosylphosphatidylinositols - metabolism; Lactobacillus plantarum; Lactobacillus plantarum - cytology; Lactobacillus plantarum - growth & development; Membrane Proteins - chemistry; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mixed-species biofilm; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Sake yeast; Awa1p; Sake yeast; Lactobacillus plantarum; Mixed-species biofilm; Co-aggregation
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2014.10.007

We examined mixed-species biofilm formation between Lactobacillus plantarum ML11-11 and both foaming and non-foaming mutant strains of Saccharomyces cerevisiae sake yeasts. Wild-type strains showed significantly lower levels of biofilm formation compared with the non-foaming mutants. Awa1p, a protein involved in foam formation during sake brewing, is a glycosylphosphatidylinositol (GPI)-anchored protein and is associated with the cell wall of sake yeasts. The AWA1 gene of the non-foaming mutant strain Kyokai no. 701 (K701) has lost the C-terminal sequence that includes the GPI anchor signal. Mixed-species biofilm formation and co-aggregation of wild-type strain Kyokai no. 7 (K7) were significantly lower than K701 UT-1 (K701 ura3/ura3 trp1/trp1), while the levels of strain K701 UT-1 carrying the AWA1 on a plasmid were comparable to those of K7. The levels of biofilm formation and co-aggregation of the strain K701 UT-1 harboring AWA1 with a deleted GPI anchor signal were similar to those of K701 UT-1. These results clearly demonstrate that Awa1p present on the surface of sake yeast strain K7 inhibits adhesion between yeast cells and L. plantarum ML11-11, consequently impeding mixed-species biofilm formation.