Lignocellulose Fermentation Products Generated by Giant Panda Gut Microbiomes Depend Ultimately on pH Rather than Portion of Bamboo: A Preliminary Study

• Published in: Microorganisms (Basel) Vol. 10; no. 5; p. 978
• Main Authors: Scoma, Alberto, Khor, Way Cern, Coma, Marta, Heyer, Robert, Props, Ruben, Bouts, Tim, Benndorf, Dirk, Li, Desheng, Zhang, Hemin, Rabaey, Korneel
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.05.2022; MDPI
• Subjects: Alcohols; alpha amylase; Bamboo; Digestion; Digestive system; Digestive tract; Dung; Ethanol; Experimentation; Feces; Feeds; Fermentation; giant panda; gut microbiome; Hemicellulose; Hypotheses; Incubation; Intestinal microflora; Lactic acid; Lignocellulose; Microbiomes; Microbiota; Nutrition; Pandas; pH effects; Wildlife conservation; α-Amylase; lactic acid; hemicellulose; meta proteomics; gut microbiome; alpha amylase; metabolomics; giant panda; fermentation; ethanol; 16S rRNA gene
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms10050978

Giant pandas feed almost exclusively on bamboo but miss lignocellulose-degrading genes. Their gut microbiome may contribute to their nutrition; however, the limited access to pandas makes experimentation difficult. In vitro incubation of dung samples is used to infer gut microbiome activity. In pandas, such tests indicated that green leaves are largely fermented to ethanol at neutral pH and yellow pith to lactate at acidic pH. Pandas may feed on either green leaves or yellow pith within the same day, and it is unclear how pH, dung sample, fermentation products and supplied bamboo relate to one another. Additionally, the gut microbiome contribution to solid bamboo digestion must be appropriately assessed. Here, gut microbiomes derived from dung samples with mixed colors were used to ferment green leaves, also by artificially adjusting the initial pH. Gut microbiomes digestion of solid lignocellulose accounted for 30-40% of the detected final fermentation products. At pH 6.5, mixed-color dung samples had the same fermentation profile as green dung samples (mainly alcohols), while adjusting the initial pH to 4.5 resulted in the profile of yellow dung samples (mainly lactate). Metaproteomics confirmed that gut microbiomes attacked hemicellulose, and that the panda's alpha amylase was the predominant enzyme (up to 75%).