A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis

• Published in: Cellular and molecular life sciences : CMLS Vol. 60; no. 8; pp. 1725 - 1732
• Main Authors: Hu, Q-D, Lu, H, Huo, K, Ying, K, Li, J, Xie, Y, Mao, Y, Li, Y-Y
• Format: Journal Article
• Language: English
• Published: Switzerland Springer Nature B.V 01.08.2003; Birkhäuser-Verlag
• Subjects: Amino Acid Sequence; Amino acids; Base Sequence; Cloning, Molecular; DNA, Complementary - genetics; Exons; Gene Expression; Genes, Fungal; Genetic Complementation Test; Humans; Introns; Molecular Sequence Data; Mutation; Phosphotransferases (Alcohol Group Acceptor) - chemistry; Phosphotransferases (Alcohol Group Acceptor) - genetics; Phosphotransferases (Alcohol Group Acceptor) - metabolism; Plasmids - genetics; Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Species Specificity; Tumor Protein, Translationally-Controlled 1
• ISSN: 1420-682X; 1420-9071
• DOI: 10.1007/s00018-003-3107-7

The Saccharomyces cerevisiae TPT1 gene plays a role in removing the 2'-phosphate from ligated tRNA during the maturation of pre-tRNA. Here we reported the cloning and characterization of the human TRPT1 gene as a homolog of yeast TPT1. The TRPT1 gene is located at human chromosome 11q13 and encodes a polypeptide of 253 amino acids. BLAST searches with its amino acid sequence revealed the ubiquitous occurrence of TRPT1 homologs and their functional relationships with the presence of the DUF60/KptA domain. Northern analysis demonstrated that the gene is primarily expressed in heart and skeletal muscle, with lower or undetectable levels in other tissues studied. A plasmid-shuffling experiment showed that the human TRPT1 gene could complement the tpt1 mutation in S. cerevisiae