Amperometric Tyrosinase Biosensors Based on Nanomaterial-Modified Electrodes for Aflatoxin M1

• Published in: Journal of analytical chemistry (New York, N.Y.) Vol. 74; no. Suppl 1; pp. 59 - 67
• Main Authors: Varlamova, R. M., Medyantseva, E. P., Khamidullina, R. R., Budnikov, H. C.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.07.2019; Springer; Springer Nature B.V
• Subjects: Aflatoxin M1; Analytical Chemistry; Biological products; Biosensors; Carbon nanotubes; Chemistry; Chemistry and Materials Science; Chitosan; Dairy products; Electrical measurement; Electrodes; Enzymes; Gold; Graphene; Medical equipment; Nanomaterials; Nanoparticles; Nanotubes; Parameter modification; Physiological apparatus; Platinum; Tyrosinase; Russia; aflatoxin M1; graphene oxide; carbon nanotubes; tyrosinase biosensor; gold nanoparticles
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1134/S1061934819070189

We propose amperometric biosensors for the determination of aflatoxin M1, based on screen-printed platinum electrodes modified by carbon nanotubes (CNT), graphene oxide (GO), gold nanoparticles (Au-NP) in chitosan, and immobilized tyrosinase. The conditions for obtaining gold nanoparticles are optimized. Aflatoxin M1 exhibits properties of a reversible tyrosinase inhibitor, which makes ensures its determination using biosensors modified by nanomaterials in the concentration range 1 × 10 –11 –1 × 10 –6 M with LOD = 5 × 10 –12 M. The kinetic studies of the enzymatic conversion of phenol in the presence of aflatoxin M1 and a tyrosinase biosensor show both two-parameter mismatch inhibition (modification with a CNT/Au-NP composite) and two-parameter coordinated inhibition (modification with GO/Au-NP). Using the proposed enzyme sensors based on tyrosinase, the procedures for determining aflatoxin M1 in dairy products are tested.