Interaction between the C. elegans cell-death regulators CED-9 and CED-4

• Published in: Nature (London) Vol. 385; no. 6617; pp. 653 - 656
• Main Authors: Spector, Mona S, Desnoyers, Serge, Hoeppner, Daniel J, Hengartner, Michael O
• Format: Journal Article
• Language: English
• Published: London Nature Publishing 13.02.1997; Nature Publishing Group
• Subjects: Ageing, cell death; Animals; Apoptosis - physiology; Apoptosis Regulatory Proteins; Binding Sites; Biological and medical sciences; Caenorhabditis elegans - metabolism; Caenorhabditis elegans - physiology; Caenorhabditis elegans Proteins; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - metabolism; Cell physiology; Cellular biology; Fundamental and applied biological sciences. Psychology; Genetics; Helminth Proteins - genetics; Helminth Proteins - metabolism; Molecular and cellular biology; Mutagenesis, Site-Directed; Protein Binding; Proteins; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; Proto-Oncogene Proteins c-bcl-2; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Saccharomyces cerevisiae - genetics; Sequence Deletion; Yeast; Caenorhabditis elegans; Regulation(control); Helmintha; Nemathelminthia; Invertebrata; Effector analog; Nematoda; Apoptosis
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/385653a0

Programmed cell death (apoptosis) is an evolutionarily conserved process used by multicellular organisms to eliminate cells that are not needed or are potentially detrimental to the organism. Members of the Bcl-2 family of mammalian proteins are intimately involved in the regulation of apoptosis, but, their precise mechanism of action remains unresolved. In Caenorhabditis elegans, the Bcl-2 homologue CED-9 prevents cell death by antagonizing the death-promoting activities of CED-3, a member of the Caspase family of death proteases, and of CED-4, a protein with no known mammalian homologue. Here we show that CED-9 interacts physically with CED-4. Mutations that reduce or eliminate CED-9 activity also disrupt its ability to bind CED-4, suggesting that this interaction is important for CED-9 function. Thus, CED-9 might control C. elegans cell death by binding to and regulating CED-4 activity. We propose that mammalian Bcl-2 family members might control apoptosis in a similar way through interaction and regulation of CED-4 homologues or analogues.