Quantification of Plant Cell Death by Electrolyte Leakage Assay

We describe a protocol to measure the electrolyte leakage from plant tissues, resulting from loss of cell membrane integrity, which is a common definition of cell death. This simple protocol is designed to measure the electrolyte leakage from a tissue sample over a time course, so that the extent of...

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Bibliographic Details
Published inBio-protocol Vol. 8; no. 5; p. e2758
Main Authors Hatsugai, Noriyuki, Katagiri, Fumiaki
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.03.2018
Bio-protocol LLC
Subjects
Biology
Methods
Electrolyte leakage
Electrolytic conductivity
Plants
Cell death
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Summary:We describe a protocol to measure the electrolyte leakage from plant tissues, resulting from loss of cell membrane integrity, which is a common definition of cell death. This simple protocol is designed to measure the electrolyte leakage from a tissue sample over a time course, so that the extent of cell death in the tissue can be monitored dynamically. In addition, it is easy to handle many tissue samples in parallel, which allows a high level of biological replication. Although the protocol is exemplified by cell death in in response to pathogen challenge, it is easily applicable to other types of plant cell death.
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ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2758