Rhodanobacter caeni sp. nov., isolated from sludge from a sewage disposal plant

• Published in: International journal of systematic and evolutionary microbiology Vol. 62; no. Pt 12; pp. 2815 - 2821
• Main Authors: Woo, Sung-Geun, Srinivasan, Sathiyaraj, Kim, Myung Kyum, Lee, Myungjin
• Format: Journal Article
• Language: English
• Published: Reading International Union of Microbiological Societies 01.12.2012; Society for General Microbiology
• Subjects: bacteria; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; DNA; DNA, Bacterial - genetics; fatty acids; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; genes; Microbiology; Molecular Sequence Data; new species; nucleotide sequences; phosphatidylethanolamines; Phospholipids - analysis; Phylogeny; Republic of Korea; Rhodanobacter; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; Sequence Homology, Nucleic Acid; sewage; Sewage - microbiology; sludge; Systematics; Ubiquinone - analysis; ubiquinones; Xanthomonadaceae - classification; Xanthomonadaceae - genetics; Xanthomonadaceae - isolation & purification; South Korea; Sewage disposal; Bacteria; New species; Taxonomy; Sewage sludge
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.033365-0

Two Gram-reaction-negative, motile bacteria, designated strains MJ01T and MJ14, were isolated from sludge collected from the Daejeon sewage disposal plant in South Korea. The taxonomic positions of both strains were determined using a polyphasic approach. In phylogenetic analyses based on 16S rRNA gene sequences, strains MJ01T and MJ14 appeared indistinguishable and to be most closely related to members of the genus Rhodanobacter in the family Xanthomonadaceae of the Gammaproteobacteria (96.4–98.8 % sequence similarity). Strain MJ01T exhibited a relatively high level of DNA–DNA relatedness with strain MJ14 (89.3 %) but relatively low DNA–DNA relatedness values with established species in the genus Rhodanobacter (<60 %). The genomic DNA G+C contents of strains MJ01T and MJ14 were 65.3 and 64.8 mol%, respectively. The major respiratory quinone of both novel strains was the ubiquinone Q-8. The major fatty acids of both strains were iso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and iso-C17 : 1ω9c, and the polar lipid profiles of the two strains contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and minor amounts of unidentified aminophospholipids and phospholipids. Based on the phenotypic, genotypic and phylogenetic evidence, strains MJ01T and MJ14 represent a single novel species in the genus Rhodanobacter, for which the name Rhodanobacter caeni sp. nov. is proposed. The type strain is MJ01T ( = KCTC 22449T = JCM 16242T), with MJ14 ( = KCTC 22460 = JCM 16243) as a reference strain.