IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis

Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocytes into the inflamed joint synovium. Interleukin (IL)-17 is a critical inflammatory mediator that participates in the progression of OA, although the mechanisms linking IL-17 and monocyte infiltration are not well unders...

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Published inInternational journal of molecular sciences Vol. 23; no. 12; p. 6804
Main Authors Wu, Tsung-Ju, Chang, Sunny Li-Yun, Lin, Chih-Yang, Lai, Chao-Yang, He, Xiu-Yuan, Tsai, Chun-Hao, Ko, Chih-Yuan, Fong, Yi-Chin, Su, Chen-Ming, Tang, Chih-Hsin
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 18.06.2022
MDPI
Subjects
adhesion
Arthritis
Biomedical materials
c-Jun protein
Cartilage
CD11b antigen
Cell adhesion
Cell adhesion & migration
Cell adhesion molecules
Cytokines
Disease
Fibroblasts
Gene expression
IL-17
Inflammation
Interleukin 17
JNK protein
Kinases
MicroRNAs
Monocytes
Osteoarthritis
Pathogenesis
Phosphorylation
Protein kinase C
Synovium
Transcription factors
Vascular cell adhesion molecule 1
VCAM-1
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Summary:Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocytes into the inflamed joint synovium. Interleukin (IL)-17 is a critical inflammatory mediator that participates in the progression of OA, although the mechanisms linking IL-17 and monocyte infiltration are not well understood. Our analysis of synovial tissue samples retrieved from the Gene Expression Omnibus (GEO) dataset exhibited higher monocyte marker (CD11b) and vascular cell adhesion molecule 1 (VCAM-1) levels in OA samples than in normal, healthy samples. The stimulation of human OA synovial fibroblasts (OASFs) with IL-17 increased VCAM-1 production and subsequently enhanced monocyte adhesion. IL-17 affected VCAM-1-dependent monocyte adhesion by reducing miR-5701 expression through the protein kinase C (PKC)-α and c-Jun N-terminal kinase (JNK) signaling cascades. Our findings improve our understanding about the effect of IL-17 on OA progression and, in particular, VCAM-1 production and monocyte adhesion, which may help with the design of more effective OA treatments.
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These authors contributed equally to this work.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23126804