Clinical outcomes after IVF or ICSI using human blastocysts derived from oocytes containing aggregates of smooth endoplasmic reticulum

• Published in: Reproductive biomedicine online Vol. 34; no. 4; pp. 337 - 344
• Main Authors: Itoi, Fumiaki, Asano, Yukiko, Shimizu, Masashi, Nagai, Rika, Saitou, Kanako, Honnma, Hiroyuki, Murata, Yasutaka
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.04.2017
• Subjects: Adult; Aggregates of smooth endoplasmic reticulum; Blastocyst - cytology; Blastocyst - ultrastructure; Clinical outcome; Embryo Transfer; Embryonic Development; Endoplasmic Reticulum, Smooth - ultrastructure; Female; Fertilization in Vitro; Humans; ICSI; IVF; Neo-natal outcome; Obstetrics and Gynecology; Oocytes - ultrastructure; Pregnancy; Pregnancy Rate; Sperm Injections, Intracytoplasmic; Vitrified–warmed blastocyst transfer; IVF; vitrified–warmed blastocyst transfer; ICSI; neo-natal outcome; aggregates of smooth endoplasmic reticulum; clinical outcome; Vitrified–warmed blastocyst transfer; Clinical outcome; Aggregates of smooth endoplasmic reticulum; Neo-natal outcome
• ISSN: 1472-6483; 1472-6491
• DOI: 10.1016/j.rbmo.2017.01.007

Abstract In this study the clinical and neo-natal outcomes after transfer of blastocysts derived from oocytes containing aggregates of smooth endoplasmic reticulum (SER) were compared between IVF and intracytoplasmic sperm injection (ICSI) cycles. Clinical and neo-natal outcomes of blastocysts in cycles with at least one SER metaphase II oocyte (SER+MII; SER+ cycles) did not significantly differ between the two insemination methods. When SER+MII were cultured to day 5/6, fertilization, embryo cleavage and blastocyst rates were not significantly different between IVF and ICSI cycles. In vitrified-warmed blastocyst transfer cycles, the clinical pregnancy rates from SER+MII in IVF and ICSI did not significantly differ. In this study, 52 blastocysts (27 IVF and 25 ICSI) derived from SER+MII were transferred, yielding 15 newborns (5 IVF and 10 ICSI) and no malformations. Moreover, 300 blastocysts (175 IVF and 125 ICSI) derived from SER‒MII were transferred, yielding 55 newborns (24 IVF and 31 ICSI cycles). Thus, blastocysts derived from SER+ cycles exhibited an acceptable ongoing pregnancy rate after IVF ( n = 125) or ICSI ( n = 117) cycles. In conclusion, blastocysts from SER+MII in both IVF and ICSI cycles yield adequate ongoing pregnancy rates with neo-natal outcomes that do not differ from SER‒MII.