Changes in the Level of Spontaneous DNA Damage in Whole Blood Leukocytes during Storage
We evaluated structural damage to DNA (%TDNA) in blood leukocytes from healthy donors of different age at different periods (0-6 days) of blood storage at 4-8°C. It was found that the basal level of DNA damage increased and intracellular antioxidant level decreased during storage. Mean %TDNA was 6.8...
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Published in | Bulletin of experimental biology and medicine Vol. 154; no. 1; pp. 37 - 39 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Boston
Springer US
01.11.2012
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | We evaluated structural damage to DNA (%TDNA) in blood leukocytes from healthy donors of different age at different periods (0-6 days) of blood storage at 4-8°C. It was found that the basal level of DNA damage increased and intracellular antioxidant level decreased during storage. Mean %TDNA was 6.8±3.3% in the fresh blood and 19.2±8.1% after 5-day storage. The experiments with exposure to reactive oxygen species induced by irradiation suggest that depletion of low-molecular-weight endogenous antioxidants occurs as soon as after 5-h storage. Our results suggest that storage time should be taken into account when assessing the basal and induced levels of leukocyte DNA damage by the comet assay. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0007-4888 1573-8221 |
DOI: | 10.1007/s10517-012-1869-3 |